DYCP-31DN Comb 13/6 amariba (1.0mm)

Sisitemu ya DYCP-31DN ni sisitemu ya electrophoreis ya horizontal.Mu buryo bwa elegitoroniki ya elegitoroniki, gele iterwa mu cyerekezo gitambitse kandi ikarohama muri buffer ikora mu gasanduku ka gel. Agasanduku ka gel kagabanyijemo ibice bibiri, hamwe na agarose gel itandukanya byombi. Nkuko byavuzwe mbere, anode iherereye kumpera imwe, mugihe cathode iherereye kurundi. Ionic ikora buffer yemerera kwishyurwa gradient kurema mugihe ikoreshwa. Mubyongeyeho, buffer ikora kugirango ikonje gel, ishyuha nkuko byishyurwa. Gukoresha buffer ikunze kuzenguruka kugirango irinde pH igipimo. Dufite ubunini butandukanye bwibimamara byo gukoresha.Ibimamara bitandukanye bituma iyi sisitemu ya electrophoreis ya horizontal iba nziza kubikorwa byose bya agarose gel harimo na electrophoreis yo mu mazi, kuri electrophoreis yihuta ifite urugero ruto, ADN, electrophoreis yo mu mazi, kugirango imenye, itandukanya kandi itegure ADN, no kuri gupima uburemere bwa molekile.

Mugihe cya electrophoreis, gele ikorwa mumashanyarazi. Inzira irimo "amariba" mato afata ibice wifuza kugerageza. Microliter nyinshi (µL) yumuti urimo ibice wifuza kugerageza bipakurura neza mumariba. Hanyuma, buffer, ikora amashanyarazi, isukwa mubyumba bya electrophorei. Ibikurikira, tray tray, irimo ibice, ishyirwa mubyumba byitondewe hanyuma ikinjizwa muri buffer. Hanyuma, urugereko rufunze kandi ingufu zarafunguwe. Anode na cathode, byakozwe numuyagankuba, bikurura ibice byashizwemo. Ibice bigenda buhoro buhoro muri gel yerekeza kumurongo utandukanye. Amashanyarazi yazimye, gel irasohoka irasuzumwa.