Akagari ka Electrophoresis ya SDS-PAGE na Blot y'Iburengerazuba

Ibisobanuro bigufi:

DYCZ-24DN ni ya poroteyine electrophoreis, naho DYCZ-40D ni iyo kwimura molekile ya poroteyine kuva muri gel ikajya muri membrane nka nitrocellulose membrane mu bushakashatsi bwa WesternBlot. Hano dufite ihuza ryiza kubakiriya bacu rishobora guhura na porogaramu uwagerageje ashobora gukoresha tank imwe yo gukoragel electrophorei, hanyuma uhinduranya module ya electrode kugirango ikore igerageza rya blotting na tank imwe DYCZ-24DN. Icyo ukeneye ni sisitemu ya DYCZ-24DN gusa wongeyeho DYCZ-40D module ya Electrode izagufasha guhinduka vuba kandi byoroshye kuva muri tekinike ya electrophoreis ujya mubindi.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Igipimo (L × W × H)	140 × 100 × 150mm
Ingano ya Gel (L × W)	75 × 83mm
Comb	Iriba 10 n'iriba 15
Kurwanya Ubunini	1.0mm na 1.5mm (Bisanzwe) 0,75mm (Bihitamo)
Umubare w'icyitegererezo	20-30
Umubumbe wa Buffer	400ml
Ibiro	1kg

Ibisobanuro

DYCZ-24DN ni selile ya vertical electrophoreis selile (tank / chamber) ya SDS-PAGE, PAGE Kavukire nibindi bya poroteyine. Iyi selile irashobora guta no gukoresha gel ahantu hamwe. Nibyoroshye kandi byihariye byoroshye kandi byoroshye gupakira ingero. Ikigega gikozwe mubikoresho byiza bya polyakarubone, biramba cyane kandi bisobanutse. Iyi tank ibonerana yorohereza kureba gel mugihe ukora igerageza. DYCZ-24DN ifite electrode ikurwaho byoroshye kubungabunga. Electrode ikorwa na platine yuzuye (≥99.95%) arizo electrolysis-ruswa kandi ihanganira ubushyuhe bwinshi.

Nyuma ya gel electrophorei, ukurikije ibisabwa mubigeragezo, rimwe na rimwe, uwagerageje akeneye kwimurira gel inkunga ikomeye kugirango isesengurwe. Yitwa blotting igeragezwa, nuburyo bwo kohereza poroteyine, ADN cyangwa RNA kubitwara. Bikorwa nyuma ya gel electrophorei, kwimura molekile ziva muri gel kuri blotting membrane. Nyuma yo guhanagura, poroteyine zimuwe, ADN cyangwa RNA noneho zigaragazwa no gusiga amabara (urugero, irangi rya feza rya poroteyine), iyerekanwa rya autoradiografique ya molekile ya radiolabelled (ikorwa mbere ya blot), cyangwa ikimenyetso cyihariye cya poroteyine cyangwa acide nucleique. Iyanyuma ikorwa na antibodies cyangwa progaramu ya Hybridisation ihuza gusa na molekile zimwe na zimwe za blot kandi zifite enzyme zifatanije nabo. Nyuma yo gukaraba neza, iki gikorwa cyimisemburo (kandi rero, molekile dushakisha muri blot) zigaragazwa nubushakashatsi hamwe nuburyo bukwiye, butanga ububiko bwamabara kuri blot cyangwa reaction ya chemiluminescent yanditswe na firime yifoto.

Kumashanyarazi kuriyi selire ihagaze ya selile electrophoreis, turasaba imwe mumashanyarazi ya timer igenzura amashanyarazi DYY-6C.

Gusaba

Kuri SDS-PAGE, Kavukire ya PAGE electrophoreis no kwimura molekile ya poroteyine kuva muri gel ikajya muri membrane.

Ikiranga

DYCZ-24DN mini vertical gel electrophoresis selile ya SDS-PAGE, Kavukire ya PAGE electrophoreis ifite ibintu bikurikira:

•Ikozwe muri polikarubone yujuje ubuziranenge, nziza kandi iramba, byoroshye kubireba;

• Hamwe na jel itera muburyo bwumwimerere, ibasha guterera no gukoresha gel ahantu hamwe, byoroshye kandi byoroshye gukora geles, kandi ukabika umwanya wawe w'agaciro;

Igishushanyo cyihariye cya wedge kirashobora gutunganya icyumba cya gel;

• Ikigega kibumbabumbwe gifite ibikoresho bya electrode nziza;

• Biroroshye kandi byoroshye kongeramo ingero;

•Bashoboye run gel imwe cyangwa geles ebyiri icyarimwe;

Kubika igisubizo cya buffer;

• Igishushanyo cyihariye cya tank wirinde kumeneka na gel;

•Gukuraho electrode, byoroshye kubungabunga no gusukura;

• Auto-off-off mugihe umupfundikizo ufunguye;

Module ya electrode, nayo yitwa Gushyigikira umubiri wo kwimura cyangwa guteranya electrode nigice cyibanze kuri blotting sisitemu DYCZ-40D. Igizwe n'ibice by'ibara ry'umutuku n'umukara hamwe na electrode itukura n'umukara kugirango harebwe icyerekezo cyiza cya gel mugihe cyo kwimura, hamwe nigishushanyo mbonera cyoroshya kwinjiza no kuvanaho kaseti zifata gel mu mubiri ushyigikirwa kugirango zimurwe (inteko ya electrode).