Gene Electroporator GP-3000

Ibisobanuro bigufi:

GP-3000 Gene Electroporator igizwe nigikoresho nyamukuru, igikombe cyo kumenyekanisha gene, hamwe ninsinga zidasanzwe zihuza.Ikoresha cyane cyane amashanyarazi kugirango yimure ADN mu ngirabuzimafatizo zibishoboye, ibimera n’inyamanswa, hamwe n’imisemburo.Ugereranije nubundi buryo, uburyo bwa Gene Introducer butanga ibyiza nko gusubiramo cyane, gukora neza, koroshya imikorere, no kugenzura umubare.Byongeye kandi, amashanyarazi nta genotoxicity afite, bituma iba tekinike yingenzi muri biologiya ya molekuline.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Icyitegererezo	GP-3000
Ifishi	Kwangirika kwangirika hamwe na Wave
Umuvuduko mwinshi mwinshi	401-3000V
Umuvuduko muke usohoka	50-400V
Umuyoboro mwinshi wa voltage	10-60μF muntambwe 1μF (10μF, 25μF, 35μF, 50μF, 60μF basabwe)
Umuyoboro muto	25-1575μF muri 1μF intambwe (25μF intambwe isabwa)
Kurwanya	100Ω-1650Ω muri 1Ω intambwe (50Ω basabwe)
Amashanyarazi	100-240VAC50 / 60HZ
Sisitemu y'imikorere	Igenzura rya microcomputer
Igihe gihoraho	hamwe na RC igihe gihoraho, kirashobora guhinduka
Uburemere bwiza	4.5kg
Ibipimo by'ipaki	58x36x25cm

Ibisobanuro

Amashanyarazi ya selile nuburyo bwingenzi bwo kwinjiza macromolecules zidasanzwe nka ADN, RNA, siRNA, proteyine, na molekile nto imbere mumbere ya selile.

Mugihe cyumuriro wamashanyarazi ukomeye kumwanya muto, selile ya selile mugisubizo yunguka neza.Ibintu byashizwemo byinjira byinjira muri selile muburyo busa na electrophorei.Bitewe nokurwanya kwinshi kwa fosifolipide bilayeri ya selile ya selile, voltage ya bipolar itangwa numuriro w'amashanyarazi wo hanze utwarwa na membrane selile, kandi voltage ikwirakwizwa muri cytoplazme irashobora kwirengagizwa, hafi ya ntamashanyarazi muri cytoplazme, bityo rero no kumenya uburozi buto murwego rusanzwe rwa electrophoreis.

Gusaba

Irashobora gukoreshwa mumashanyarazi kugirango yimure ADN mungirabuzimafatizo zibishoboye, ibimera ninyamaswa, hamwe ningirabuzimafatizo.Nk’amashanyarazi ya bagiteri, umusemburo, nizindi mikorobe, kwanduza ingirangingo z’inyamabere, no kwanduza ingirangingo z’ibimera na protoplasts, kuvanga ingirabuzimafatizo no kumenyekanisha gene, kwinjiza genes zerekana ibimenyetso byerekana ibimenyetso, kwinjiza ibiyobyabwenge, poroteyine, antibodies, nizindi molekile zo kwiga imiterere yimikorere nimikorere.

Ikiranga

• Gukora neza: igihe gito cyo guhinduka, igipimo kinini cyo guhinduka, gusubiramo cyane;

• Ububiko bwubwenge: bushobora kubika ibipimo byubushakashatsi, byorohereza abakoresha gukora;

• Igenzura risobanutse: microprocessor igenzurwa na pulse isohoka; Ø

• Isura nziza: igishushanyo mbonera cyimashini yose, kwerekana intiti, imikorere yoroshye.

Ibibazo

Ikibazo: Electroporator ni iki?

Igisubizo: Gene Electroporator ni igikoresho gikoreshwa mugutangiza ibintu bikomoka ku ngirabuzima fatizo nka ADN, RNA, na poroteyine, mu ngirabuzimafatizo binyuze mu nzira y'amashanyarazi.

Ikibazo: Ni ubuhe bwoko bw'utugingo dushobora kwibasirwa na Electroporator ya Gene?

Igisubizo: Electroporator ya Gene irashobora gukoreshwa mugutangiza ibintu bikomoka mubwoko butandukanye burimo bagiteri, umusemburo, ingirabuzimafatizo, ingirangingo z’inyamabere, nizindi mikorobe.

Ikibazo: Ni ubuhe buryo bukoreshwa bwa Gene Electroporator?

• Amashanyarazi ya bagiteri, umusemburo, nizindi mikorobe: Kubijyanye no guhindura imiterere no kwiga imikorere ya gene.

• Ihererekanyabubasha ry’inyamabere, inyama z’ibimera, hamwe na protoplasts: Kubisesengura ryerekana imiterere ya gen, imikorere yimikorere, hamwe nubuhanga bwa geneti.

• Kuvanga ingirabuzimafatizo no kumenyekanisha gene: Kubyara ingirabuzimafatizo no kumenyekanisha ingirabuzimafatizo.

• Kumenyekanisha ibimenyetso bya gen: Kubirango no gukurikirana imvugo ya gene muri selile.

• Kumenyekanisha ibiyobyabwenge, poroteyine, na antibodi: Kubushakashatsi bwimiterere yimikorere nimikorere, gutanga ibiyobyabwenge, hamwe nubushakashatsi bwakozwe na poroteyine.

Ikibazo: Nigute amashanyarazi akora?

Igisubizo: Gene Electroporator ikoresha impiswi ngufi, nini cyane yumuriro wamashanyarazi kugirango habeho imyenge yigihe gito mumyanya ndangagitsina, ituma molekile zidasanzwe zinjira muri selile.Akagari ka selile gasa nyuma yumuriro wamashanyarazi, ugafata molekile zinjiye imbere muri selile.

Ikibazo: Ni izihe nyungu zo gukoresha Gene Electroporator?

Igisubizo: Gusubiramo cyane no gukora neza, koroshya imikorere: Uburyo bworoshye kandi bwihuse, kugenzura ingano, nta genotoxicity: Kwangirika kwinshi kwangiza ingirabuzimafatizo.

Ikibazo: Ese amashanyarazi ya Gene ashobora gukoreshwa muburyo bwose bwubushakashatsi?

Igisubizo: Mugihe Gene Electroporator ihindagurika, imikorere yayo irashobora gutandukana bitewe nubwoko bwakagari nibikoresho byerekanwa.Ni ngombwa guhuza imiterere kuri buri igeragezwa ryihariye.

Ikibazo: Ni ubuhe buryo bwihariye bukenewe nyuma yo gutangira?

Igisubizo: Kwiyitaho nyuma yintangiriro birashobora gushiramo ingirabuzimafatizo muburyo bwo kugarura kugirango zibafashe gusana no gusubukura imirimo isanzwe.Ibisobanuro birashobora gutandukana bitewe nubwoko bwakagari nubushakashatsi.

Ikibazo: Hari ibibazo byumutekano bijyanye no gukoresha Gene Electroporator?

Igisubizo: Ibikorwa bisanzwe byumutekano wa laboratoire bigomba gukurikizwa.Gene Electroporator ikoresha voltage nyinshi, bityo rero hagomba kubahirizwa uburyo bukwiye hamwe n’umutekano kugirango hirindwe ingaruka z’amashanyarazi.