Agarose Gel Electrophoresis ya RNA

Ubushakashatsi bushya bwa RNA

Vuba aha, ubushakashatsi bwerekanye ko ubwoko butandukanyeibyo bigabanya urwego rwo guhindura RNA ikubye kabiri ifitanye isano na autoimmune hamwe nubudahangarwa bw'umubiri.

Molekile ya RNA irashobora guhinduka. Kurugero, nucleotide irashobora kwinjizwamo, gusibwa, cyangwa guhinduka. Bumwe mu bwahinduwe cyane, ubushakashatsi bushya bwerekana ko bugira uruhare runini mugutangira indwara yumuriro, ni uguhindura nucleotide adenosine muri inosine muri RNA ikubye kabiri. Ubushakashatsiyaribyatangajweon Tariki ya 3 Kanama muri Kamere igaragaza ko ubwoko bw’imiterere bugabanya iri hinduka ryihariye bifitanye isano n’ubwiyongere bw’indwara ziterwa na autoimmune hamwe n’indwara ziterwa n’umudugudu nka psoriasis, indwara zifata amara, na diyabete yo mu bwoko bwa 1. Abanditsi bavuga ko poroteyine ya sensor ishobora kuba yibeshya kuri RNAs zidakosowe kuri molekile zamahanga, bigatuma habaho igisubizo kibabaza.

Umuhanga mu binyabuzima wa molekuline yatekereje ko's mubyukuri intambwe ikomeye, kandi yabonetse"biratangaje" uburyo guhindura RNA bigaragara ko ari ingirakamaro ku ndwara zanduza, ugereranije n'imvugo ya gene cyangwa gutera.

Nukuri mubushakashatsi bukomeye. Nkibicuruzwa bya electrophoreis bigurishwa, mfite amatsiko yo kumenya RNA niki, nuburyo abahanga basesengura RNA na electrophorei? Nubumenyi rwose bwumwuga kandi butamenyerewe kuri njye ariko burigihe birashimishije kubishakisha.

RNA ni iki?

RNA, ngufi kuri aside ya Ribonucleic, aside nucleic iba muri selile zose nzima. Bitandukanye na ADN, RNA ni imwe rukumbi. Inshingano zayo nyamukuru ni ugukora nk'intumwa itwara amabwiriza ya ADN yo kugenzura intungamubiri za poroteyine, nubwo muri virusi zimwe na zimwe RNA aho kuba ADN zitwara amakuru ya geneti.

Agarose Gel Electrophoresis ya RNA

Abantu barashoboragusuzuma ubuziranenge bwa RNA na gel electrophorei.

Beijing Liuyi Biotechnology Co.Ltd, auruganda rwumwuga rwibanda ku bicuruzwa bya electrophoreis mu myaka 50, rutanga urutonde rwibikoresho bya electrophoreis (chambers / selile) kuri gel electrophorei, nka tanki ya electrophoreis ya horizontal (chambers / selile) kuri agarose gel electrophoreis, na tanki ya vertical electrophoreis (chambers / selile) kuri protein gel electrophorei. Hagati aho, itanga kandi ubwoko butandukanye bwa electrophoreis itanga amashanyarazi, abaministri b'umwijima wisesengura hamwe na UV transilluminator. Urashobora guhitamo Beijing Liuyi's tanki ya electrophoreis (chambers / selile) gupima uburemere bwa molekuline ya RNA no gutandukanya RNA yubunini butandukanye kuri laboratoire yawe.

Hano tuzamenyekanisha ubwoko bumwe bwa horizontal electrophoresis tank (chamber / selile) moderi DYCP-31DN duhereye kubicuruzwa 31 byuruhererekane.

DYCP-31DN nubunini buke bwa horizontal electrophoreis (chamber / selile) yo gupima uburemere bwa molekile ya RNA cyangwa ADN no kubitandukanya.