1. Ibyiciro
Gel electrophorei igabanyijemo ubwoko buhagaritse (harimo geles yinkingi na geli ya slab) nubwoko butambitse (cyane cyane gela) (Ishusho 6-18). Mubisanzwe, gutandukana guhagaritse gusumba gato gutambitse, ariko gutegura geli ya horizontal bifite byibura ibyiza bine: hari inkunga munsi ya gel yose, yemerera gukoresha agarose yibanda cyane; birashoboka gutegura agarose gel plaque yibisobanuro bitandukanye; gutegura gel hamwe nicyitegererezo cyo gupakira biroroshye; urugereko rwa electrophoreis rworoshye kubaka kandi ruhendutse. Kubera ko amashanyarazi atambitse akorwa hamwe na plaque ya agarose yuzuye yuzuye hafi ya 1mm munsi yubuso bwa buffer ya electrophoreis, nanone yitwa electrophoreis yarohamye.
Electrophoresis Tank DYCP-31DN ya agarose gel electrophorei
Sisitemu ya Buffer
Mu gutandukanya aside nucleic, sisitemu nyinshi zifata sisitemu zihoraho. Ububiko bukoreshwa cyane muri electrophoreis harimo TBE (0.08mol / L Tris · HCl, pH 8.5, 0.08mol / L aside boric, 0.0024mol / L EDTA) buffer na THE (0.04mol / L Tris · HCl, pH 7.8, 0.02mol / L sodium acetate, 0.0018mol / L EDTA) buffer. Ubusanzwe buffers zitegurwa nkibisubizo 10x byimigabane kandi bigahuzwa nibisabwa mugihe ikoreshwa. Igipimo cyimuka cya ADN yumurongo nizunguruka muri agarose gel iratandukanye na buffer yakoreshejwe. Muri buffer, igipimo cyimuka cya ADN y'umurongo iruta icya ADN izenguruka, mugihe muri buffer ya TBE, ibinyuranye nukuri.
3.Gutegura Agarose Gel
(1) Gutegura Horizontal Agarose Gel
(a) Tegura intumbero ikenewe ya agarose gel ukoresheje 1x electrophoreis buffer.
. Hisha igisubizo cya agarose kuri 55 ° C hanyuma wongereho irangi rya etidium bromide (EB) kumurongo wanyuma wa 0.5 μg / ml.
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. Reka bikomere bisanzwe mubushyuhe bwicyumba.
(e) Witonze ukureho ibimamara nyuma yo gukomera byuzuye. Ongeramo urugero rukwiye rwa buffer ya electrophoreis kuri tank ya gel, urebe ko isahani ya geli yarohamye nka mm 1 munsi yubuso bwa electrophoreis.
(2) Gutegura Vertical Agarose Gel
(a) Kuraho amavuta cyangwa ibisigazwa mubisahani ukaraba hamwe na Ethanol.
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(e) Shyiramo ibimamara, wirinde kugwa mu kirere munsi y amenyo yikimamara. Rimwe na rimwe, iminkanyari irashobora kugaragara kumenyo yikimamara mugihe cyo gukonjesha agarose; mubihe nkibi, ongeraho gusa agarose yashonze hejuru kugirango ushimangire.
(f) Kuraho ikimamara. Kugira ngo wirinde kumeneka kwa bffer ahantu hapakirwa, funga isano iri hagati ya plaque ya agarose na chambre ya electrophoreis hamwe na agarose 2% hanyuma wongereho umubare ukenewe wa buffer.
(g) Ongeramo 1x electrophoreis buffer muri gel gel.
(h) Witonze witonze ingero za ADN kuri gel ya agarose munsi ya buffer.
Andi makuru yubumenyi bwibanze kuri agarose gel electrophorei, tuzabagezaho icyumweru gitaha. Wifurije aya makuru agufasha mubigeragezo byawe.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ifite ubuhanga mu gukora ibikoresho bya electrophoreis mu myaka irenga 50 hamwe nitsinda ryacu ryubuhanga ryumwuga hamwe nikigo cya R&D. Dufite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe ninkunga yo kwamamaza. Ibicuruzwa byacu byingenzi ni Akagari ka Electrophoresis (tank / chamber), Amashanyarazi ya Electrophoresis, Ubururu LED Transilluminator, UV Transilluminator, Gel Image & Analyse Sisitemu nibindi.
Ubu turimo gushakisha abafatanyabikorwa, ikigega cya OEM electrophoresis hamwe nababitanga barahawe ikaze.
Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.
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Igihe cyo kohereza: Ukuboza-07-2023