Ubuhanga bwibanze bwa Agarose Gel Electrophoresis (2)

Icyitegererezo cyo Gutegura naKuremera

Bitewe no gukoresha sisitemu ikomeza ya buffer idashyizeho gel mu bihe byinshi, ingero zigomba kugira kwibanda hamwe nubunini buke. Koresha aumuyoborokongeramo buhoro icyitegererezo, hamwe na 5-10 μg kuri buri riba, kugirango wirinde kugabanuka gukomeye mubisubizo. Igiheicyitegererezo, amashanyarazi agomba kuzimwa. Icyitegererezo kigomba kuba kirimo irangi ryerekana (0,025% bromophenol yubururu cyangwa orange) na sucrose (10-15%) cyangwa glycerol (5-10%) kugirango byongere ubwinshi bwabyo, kwibanda ku cyitegererezo, no kwirinda gukwirakwizwa. Nyamara, rimwe na rimwe sucrose cyangwa glycerol birashobora gutera U-shusho ya U mubisubizo bya electrophoreis, bishobora kwirindwa ukoresheje Ficoll 2,5% (polyvinylpyrrolidone).

Amashanyarazi

Umuvuduko wa electrophoreis ni 5-15 V / cm, muri rusange hafi 10 V / cm. Kubitandukanya molekile nini, voltage igomba kuba munsi, mubisanzwe itarenze 5 V / cm.

Kwanduza

Fluorescent dye etidium bromide (EB) ikoreshwa muburyo bwo gusiga irangi rya ADN muri gel ya agarose. EB irashobora gushyiramo ibice bibiri bya molekile ya ADN, bigatuma EB ihuza na ADN. Kwinjira k'umucyo wa ultraviolet 260 nm na ADN byimurirwa muri EB, naho EB ihambiriye isohora fluorescence kuri 590 nm mukarere gatukura-orange k'urumuri rugaragara. Gusiga gel mu gisubizo cya 1 mmol / L MgSO4 kumasaha 1 birashobora kugabanya fluorescence yibanze iterwa na EB idafunze, byoroha kumenya ADN nkeya.

Irangi rya EB rifite ibyiza byinshi: biroroshye gukoresha, ntibimena aside nucleic, bifite sensibilité nyinshi, kandi birashobora kwanduza ADN na RNA. EB irashobora kongerwaho icyitegererezo kandi igakurikiranwa ukoresheje UV iyinjiza igihe icyo aricyo cyose. Nyuma yo kwanduza, EB irashobora gukurwaho no gukuramo n-butanol.

Nyamara, irangi rya EB ni mutagen ikomeye, kandi ingamba zo kwirinda, nko kwambara gants za polyethylene, zigomba gufatwa mugihe cyo gukora. Icunga rya Acridine naryo ni irangi risanzwe rikoreshwa kuko rishobora gutandukanya acide nucleic acide imwe-imwe (ADN, RNA). Yerekana icyatsi kibisi (530 nm) kuri acide nucleic acide ebyiri na fluorescence itukura (640 nm) kuri acide nucleic imwe imwe. Byongeye kandi, andi marangi nka methylene yubururu, methylene icyatsi, na quinoline B arashobora gukoreshwa mugusiga irangi.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ifite ubuhanga mu gukora ibikoresho bya electrophoreis mu myaka irenga 50 hamwe nitsinda ryacu ryubuhanga ryumwuga hamwe nikigo cya R&D. Dufite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe ninkunga yo kwamamaza. Ibicuruzwa byacu byingenzi ni Akagari ka Electrophoresis (tank / chamber), Amashanyarazi ya Electrophoresis, Ubururu LED Transilluminator, UV Transilluminator, Gel Image & Analyse Sisitemu nibindi.

Ubu turimo gushakisha abafatanyabikorwa, ikigega cya OEM electrophoresis hamwe nababitanga barahawe ikaze.

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.

Nyamuneka Sikana kode ya QR kugirango wongere kuri Whatsapp cyangwa WeChat.