Cellulose Acetate Membrane Electrophoresis

Cellulose Acetate Membrane Electrophoresis ni iki?

Cellulose Acetate Membrane Electrophoresis ni ubwoko bumwe bwa tekinike ya electrophoreis ikoresha selulose acetate membrane nkigitangazamakuru gishyigikira ubushakashatsi.

Cellulose Acetate ni ubwoko bwa acetate ya selile ikomoka kuri hydroxyl ya selile. Iyo ikemutse mubisubizo kama nka acetone, irashobora gushirwa kuri firime imwe kandi nziza hamwe na micro-pores. Ubunini bwa firime buri hafi 0.1-0.15mm ikwiranye nubushakashatsi.

Ibyiza bya selulose acetate membrane

Ugereranije nimpapuro za fliter, ifite ibyiza bikurikira:

1.Ingaruka nziza yo gutandukana. Kuri poroteyine ntangarugero, selulose acetate membrane ikuramo bike muri byo, nyuma yo gusiga irangi, irashobora gushushanya rwose uhereye ibara ryinyuma. Irangi ryirangi rirasobanutse neza, bityo riteza imbere ukuri kugena umubare.
2.Byihuta kandi ubike umwanya. Indwara ya selulose acetate ntishobora kuba hydyophilique kuruta impapuro za fliter, bityo imikorere ya electro-osmose ntabwo iri munsi yimpapuro za fliter, kandi umuyagankuba ukorwa cyane cyane nicyitegererezo gishobora kugabanya igihe cyo gutandukana kandi ntigabanye igihe cya electrophoreis. Mubisanzwe, selulose acetate membrane electrophoresis igihe ni 45-60minute. Inzira yose ya electrophoreis harimo gusiga irangi no gushushanya ikenera iminota 90 gusa.
3.Uburemere bukabije hamwe nicyitegererezo gito cyo gukoreshwa. Irakeneye serumu 2 μL gusa muri serumu protein electrophoreis.
4.Ibisabwa byinshi. Zimwe muri poroteyine ntabwo byoroshye gutandukanya impapuro za electrophoreis, nka Alpha-fetoprotein, enzyme ya bacteriolytic, insuline, na histone nibindi. Ariko nibyiza cyane gukoresha selile ya acetate membrane electrophoreis kugirango ubatandukanye.
5.Byoroshye kubika no kugereranya. Nyuma yo gusiga irangi rya selulose acetate membrane electrophoresis, shira ingero mumuti uvanze ya acide glacial acetic acide na Ethanol kugirango ukore isahani yumye ibitse ikizwa igihe kirekire.

Kuberako selulose acetate membrane electrophoresis yoroshye gukora, ubu irakoreshwa cyane mugusesengura no gupima proteine ​​plasma, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, aside nucleic nibindi biomacromolecule.

Gutegura Cellulose Acetate Membrane Electrophoresis

Ibikoresho byakoreshejwe birimo amashanyarazi make, amashanyarazi ya horizontal. Itangazamakuru rishyigikira ni selile ya acetate membrane.

Reagents:

1.pH8.6 igisubizo cya barbitol acid Acide Diethyl barbituric, Diethyl sodium pentobarbital;
2.Stain ： Ponceau S, Trichloroacetic;
3.TBS / T cyangwa PBS / T: 95% Ethanol, acide glacial acetic;
4.Gukemura igisubizo: Ethanol ya anhydrous, acide glacial acetic.

Beijing Liuyi Biotechnology Co, ltd ifite sisitemu ikuze ya selile ya acetate membrane electrophoresis. Icyitegererezo DYCP-38C nicyumba cyoroshye cyimpapuro na slide electrophoreis, na selile ya acetate membrane electrophoresis. Umubiri nyamukuru ubumbabumbwe, nta kintu gisohoka. Moderi yo gutanga amashanyarazi DYY-2C, DYY-6C, DYY-6D, DYY-8C, DYY-10C na DYY-12 irashobora gutanga amashanyarazi kuri DYCP-38C. Mubisanzwe, umukiriya akunda guhitamo icyitegererezo DYY-6C nkamashanyarazi ya DYCP-38C.

Nkibicuruzwa nkenerwa kuri tanki ya DYCP-38C, Liuyi nayo itanga selile ya acetate ya selile. Hariho ubunini butatu busanzwe bwa membrane, 7 * 9cm, 2 * 8cm na 12 * 8cm, kandi turashobora gutanga ubunini bwihariye bwa selile ya acetate ya selile nkuko ubisabwa kimwe.

Inyandiko zerekeye igerageza:

1.Kuramo icyitegererezo kuruhande: 1-2UL irakwiriye
2.Imashanyarazi ihoraho: imbaraga zubu 0.4 ～ 0,6m A / cm
3.pH8.6 buffer ya barbitol igisubizo: Imbaraga za Ionic 0.06
4.Igihe cyo gusiga: iminota 5 ihagije
5.Kuzigama: shyira ikarita ya elecrtophoresis yumye mugusukura muminota 10-15, hanyuma uyikuremo uyishyire kumirahuri isukuye, imaze gukama, izahinduka ikarita ya firime iboneye.

Ikirangantego cya Liuyi gifite amateka arenga imyaka 50 mubushinwa kandi isosiyete irashobora gutanga ibicuruzwa bihamye kandi byujuje ubuziranenge kwisi yose.Mu myaka yiterambere, birakwiye ko uhitamo!

Kubindi bisobanuro kuri twe, nyamuneka twandikire ukoresheje imeri[imeri irinzwe], [imeri irinzwe].