Comet Assay (Akagari kamwe ka Gel Electrophoresis, SCGE) nubuhanga bworoshye kandi bwihuse bukoreshwa cyane cyane mukumenya kwangirika kwa ADN no gusana muri selile zitandukanye. Izina "Comet Assay" rituruka kumiterere iranga comet isa nibisubizo: nucleus ya selile ikora "umutwe," mugihe ibice bya ADN byangiritse byimuka, bigakora "umurizo" bisa na comet.
Ihame
Ihame rya Comet Assay rishingiye ku kwimuka kwa ADN mu muriro w'amashanyarazi. ADN yuzuye iguma muri nucleus selile, mugihe ADN yangiritse cyangwa yacitsemo ibice yimuka yerekeza kuri anode, ikora "umurizo". Uburebure nuburemere bwumurizo biragereranywa nuburyo ADN yangiritse.
Inzira
- Gutegura Akagari: Ingirabuzimafatizo zigomba gupimwa zivanze na-agarose-ya-agarose-hanyuma igakwirakwira kuri microscope kugirango ikore urwego rumwe.
- Lysis: Amashusho yibizwa mumuti wa lysis kugirango ukureho selile selile na membrane nucleaire, ugaragaza ADN.
- Amashanyarazi: Amashusho ashyirwa mubyumba bya electrophoreis munsi ya alkaline cyangwa itabogamye. Ibice bya ADN byangiritse byimukira kuri electrode nziza bitewe n'umuriro w'amashanyarazi.
- Kwanduza: Nyuma ya electrophorei, amashusho yandujwe irangi rya fluorescent (urugero, etidium bromide) kugirango yerekane ADN.
- Isesengura rya Microscopique: Ukoresheje microscope ya fluorescence cyangwa software yihariye, isesengura rya comet rirasesengurwa, kandi ibipimo nkuburebure bwumurizo nuburemere byapimwe.
Ishusho kuva biorender
Isesengura ryamakuru
Ibisubizo bivuye muri Comet Assay birasuzumwa hashingiwe kubintu byinshi byingenzi:
- Uburebure bwumurizo: Yerekana intera ADN yimuka, byerekana urugero ADN yangiritse.
- Umurizo wa ADN: Ijanisha rya ADN ryimukira murizo, akenshi rikoreshwa nkigipimo cyo kwangiza ADN.
- Umwanya wumurizo wa Olive (OTM): Ihuza uburebure bwumurizo hamwe numurizo wa ADN kugirango itange urugero rwuzuye rwangirika kwa ADN.
Porogaramu
- Ubushakashatsi bwa Genotoxicity: Comet Assay ikoreshwa cyane mugusuzuma ingaruka zimiti, ibiyobyabwenge, nimirasire kuri ADN selile, bikaba igikoresho cyingenzi cyo gupima genotoxicity.
- Uburozi bwibidukikije: Ifasha mu gusesengura ingaruka zangiza ibidukikije kuri ADN y’ibinyabuzima, itanga ubumenyi ku mutekano w’ibidukikije.
- Ubushakashatsi mu buvuzi no mu mavuriro: Comet Assay ikoreshwa mukwiga uburyo bwo gusana ADN, kanseri, nizindi ndwara ziterwa na ADN. Irasuzuma kandi ingaruka zo kuvura kanseri nka radiotherapi na chimiotherapie kuri ADN.
- Ubumenyi bwibiryo nubuhinzi: Byakoreshejwe mugusuzuma umutekano wimiti yica udukoko, inyongeramusaruro, nibindi bintu, no kwiga ingaruka zuburozi mubyitegererezo byinyamaswa.
Ibyiza
- Kumva neza: Irashoboye kumenya urwego ruke rwa ADN yangiritse.
- Igikorwa cyoroshye: Tekinike iroroshye, ituma iboneka neza-yerekana neza.
- Porogaramu Yagutse: Irashobora gukoreshwa muburyo butandukanye, harimo inyamanswa n'ibimera.
- Inzitizi: Mugihe utanga amakuru yujuje ibyangiritse kuri ADN, isesengura ryinshi rishingiye cyane kuri software hamwe nubuhanga bwo gusesengura amashusho.
- Ibigeragezo: Ibisubizo birashobora guterwa nibintu nka electrophoreis igihe na pH, bisaba kugenzura neza imiterere yubushakashatsi.
Imipaka
Comet Assay nigikoresho ntagereranywa mubushakashatsi bwibinyabuzima, siyanse y’ibidukikije, no guteza imbere ibiyobyabwenge bitewe n’ubworoherane ndetse n’ubukangurambaga bukabije mu kumenya ibyangiritse kuri ADN no kuyisana. Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology)itanga horizontal electrophoreis chambre ya comet assay. Murakaza neza kuvugana natwe kugirango tuganire kubyerekeyeKuzaprotocole.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ifite ubuhanga mu gukora ibikoresho bya electrophoreis mu myaka irenga 50 hamwe nitsinda ryacu ryubuhanga ryumwuga hamwe nikigo cya R&D. Dufite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe ninkunga yo kwamamaza. Ibicuruzwa byacu byingenzi ni Akagari ka Electrophoresis (tank / chamber), Electrophoresis Amashanyarazi, Ubururu LED Transilluminator, UV Transilluminator, Gel Image & Analyse Sisitemu nibindi kandi dutanga ibikoresho bya laboratoire nkibikoresho bya PCR, mixer ya vortex na centrifuge ya laboratoire.
Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.
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Igihe cyo kohereza: Nzeri-20-2024