Ihame ry'igerageza
Hemoglobine electrophoresis igamije kumenya no kwemeza hemoglobine zitandukanye zisanzwe kandi zidasanzwe.
Bitewe nuburyo butandukanye hamwe ningingo ya isoelectric yubwoko butandukanye bwa hemoglobine, mugisubizo runaka cya pH buffer, mugihe ingingo ya isoelectric ya hemoglobine iri munsi ya pH yumuti wa buffer, hemoglobine itwara umuriro mubi hanyuma ikimukira kuri anode mugihe cya electrophorei. Ibinyuranye, hemoglobine hamwe nuburyo bwiza bwerekeza kuri cathode.
Munsi ya voltage runaka na nyuma yigihe runaka cya electrophoreis, hemoglobine hamwe nuburemere butandukanye hamwe nuburemere bwa molekile byerekana icyerekezo cyimuka n'umuvuduko. Ibi bituma habaho gutandukanya uturere dutandukanye, hanyuma isesengura rya colimetric cyangwa electrophoretic scanning irashobora gukorerwa kuri utwo turere kugirango tumenye hemoglobine zitandukanye. Uburyo bukoreshwa cyane ni pH 8.6 selulose acetate membrane electrophoresis.
Muri cytoplazme, amatsinda ya Ethylene glycol (CHOH-CHOH) aboneka mubintu bya glycogene cyangwa polysaccharide (nka mucopolysaccharide, mucoproteine, glycoproteine, glycolipide, nibindi) bihindurwamo okiside na aside ya aldehyde (CHO-CHO). Aya matsinda ya aldehyde ahuza hamwe na reagent itagira ibara ryijimye-umutuku Schiff reagent, ikora irangi ryumutuku-umutuku ubika aho polysaccharide iba muri selire. Iyi myitwarire izwi nka aside irike-Schiff (PAS) irangi, yahoze yitwa glycogene.
Uburyo bw'igerageza
Ibikoresho:Acetate ya selilemembrane, ibikoresho bya electrophorei(DYCP-38C no gutanga amashanyarazi DYY-6C), Igikoresho cyo hejuru cyo gupakira ibikoresho (umuyoboro), spekitifotometero, amabara ya cuvettes, buffers.
Buffer:
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Inzira:
Pindishyi z'umuti wa Hemoglobin
Fata ml 3 yamaraso arimo heparin cyangwa sodium citrate nka anticoagulant. Centrifuge kuri 2000 rpm kuminota 10 hanyuma ujugunye plasma. Karaba uturemangingo twamaraso dutukura inshuro eshatu hamwe na saline physiologique (750 rpm, iminota 5 centrifugation buri gihe). Centrifuge saa 2200 rpm kuminota 10 hanyuma ujugunye ndengakamere. Ongeramo umubare ungana w'amazi yatoboye, hanyuma ongeramo inshuro 0.5 ingano ya karubone tetrachloride. Shyira imbaraga muminota 5, hanyuma centrifuge saa 2200 rpm kuminota 10 kugirango ukusanyirize Hb igisubizo cyo hejuru kugirango ukoreshwe nyuma.
Kunywa Membrane
Kata selulose acetate membrane mubice bipima cm 3 × cm 8. Wibike muri pH 8.6 TEB buffer kugeza yuzuye, hanyuma ukureho hanyuma uhanagure byumye hamwe nimpapuro.
Ikibanza
Koresha umuyoboro kugirango ubone 10 μl yumuti wa hemoglobine uhagaritse kuri selulose acetate membrane (uruhande rukomeye), hafi cm 1.5 uvuye kumpera.
Amashanyarazi
Suka igisubizo cya borate muri chambre ya electrophoreis. Shira selulose acetate membrane hamwe kuruhande rugaragara kuri cathode kumpera yicyumba. Kwiruka kuri 200 V muminota 30.
Gukuraho
Kata zone ya HbA na HbA2, ubishyire mubituba bitandukanye, hanyuma wongeremo ml 15 na ml 3 z'amazi yatoboye. Witonze witonze kugirango ukureho hemoglobine rwose, hanyuma uvange.
Ibara
Zeru kwinjiza ukoresheje amazi yatoboye kugirango igisubizo kibeho kandi upime kwinjiza kuri 415 nm.
Kubara
HbA2 (%) = Gukuramo umuyoboro wa HbA2 / (Gukuramo umuyoboro wa HbA × 5 + Gukuramo umuyoboro wa HbA2) × 100%
Kubara Ibisubizo Byubushakashatsi
Urutonde rwerekana pH 8.6 TEB Buffer Cellulose Acetate Electrophoresis: HbA> 95%, HbA2 1% -3.1%
Inyandiko
Igihe cya electrophoreis ntigikwiye kuba kirekire. Indwara ya selulose acetate ntigomba gukama mugihe cya electrophorei. Hagarika electrophoreis mugihe HbA na HbA2 batandukanijwe neza. Kumara igihe kirekire bya electrophoreis birashobora gutera gukwirakwiza no guhuzagurika.
Irinde gukoresha urugero rwinshi. Amazi menshi ya hemoglobine arashobora kuganisha kumurongo cyangwa kwanduza bidahagije, bigatuma urwego rwa HbA ruzamuka.
Irinde kwanduza selulose acetate membrane hamwe na proteyine.
Ibiriho ntibigomba kuba hejuru cyane; bitabaye ibyo, bande ya hemoglobine ntishobora gutandukana.
Buri gihe shyiramo ingero zabantu basanzwe nibikenewe bizwi na hemoglobine idasanzwe nkigenzura.
Beijing Liuyi Biotechnology ikora ikigega cya electrophoresis cyumwuga kuri hemoglobine electrophoresis nicyitegererezoDYCP-38Cselulose acetate membrane electrophoresis tank, kandi hariho moderi ebyiri zamashanyarazi ya electrophoreis aboneka kubigega bya selile ya acetate membrane electrophoresisDYY-2CnaDYY-6Camashanyarazi.
Hagati aho, Beijing Liuyi Biotechnology itanga selile ya acetate ya selile kubakiriya, kandi ingano ya selile ya acetate irashobora gutegurwa. Murakaza neza kugirango mutubaze ingero nandi makuru.
Ikirango cya Beijing Liuyi gifite amateka yimyaka irenga 50 mubushinwa kandi isosiyete irashobora gutanga ibicuruzwa bihamye kandi byujuje ubuziranenge kwisi yose. Binyuze mumyaka yiterambere, birakwiye guhitamo!
Ubu turimo gushakisha abafatanyabikorwa, ikigega cya OEM electrophoresis hamwe nababitanga barahawe ikaze.
Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221
Igihe cyo kohereza: Nzeri-20-2023