Agarozagel eYamazaki
Agarose gel electrophorei nuburyo bwa gel electrophorei ikoreshwa mubinyabuzima, ibinyabuzima bya molekuline, genetiki, na chimie clinique kugirango itandukane abaturage bavanze na macromolecules nka ADN cyangwaRNA.Ibi bigerwaho no kwimura molekile ya acide nucleic yuzuye nabi binyuze muri matrise ya agarose hamweamashanyarazi.
Ibyiza bya agarose gel
Agarose gel ni matrice-itatu igizwe na molekile ya agarose ya helical mumigozi irenze urugero yegeranijwe mubice bitatu bifite imiyoboro hamwe nu byobo bishobora kunyuramo. Imiterere ya 3-D ifatanyirizwa hamwe na hydrogène bityo bikaba bishobora guhungabana. mu gushyushya gusubira mumazi. Ubushyuhe bwo gushonga butandukanye nubushyuhe bwa gelling, ukurikije inkomoko, gel agarose ifite ubushyuhe bwa geli ya 35-42 ° C n'ubushyuhe bwa 85-95 ° C. Gushonga gake hamwe na agarose nkeya ikozwe muburyo bwo guhindura imiti nayo irahari.
Ibyiza nibibi bya agarose gel electrophorei
Inyungu nyamukuru yubuhanga bwa agarose gel electrophoresis nuko ishobora gutunganywa byoroshye kandi molekile ya ADN ikoreshwa nkicyitegererezo nayo ishobora kugarurwa nta kibi cyangiritse kurangiza inzira. Kuberako agarose gel idakora't irimo sulfate na carboxyl group, ntabwo bizatera electroosmose ya agar. Hamwe nimiterere imwe nubunini bunini bwa pore, gel ya agarose nibyiza kurigutandukanya molekile nini za ADN, nka enzyme igoye, aside nucleic na virusi. Agarose gel ntabwo't ikurura urumuri rwa UV, kuburyo rushobora kwanduzwa mu buryo butaziguye cyangwa nyuma yo guterwa kuba firime, birashobora guhinduka nanone.
Hariho kandi ibibi bimwe bya tekinike ya agarose gel electrophorei. Gel ya Agarose ivunika byoroshye, kubwibyo ubwinshi ntibushobora kuba hasi cyane, bizatwara agarose nyinshi.Agarose gel biragoye kuzigama kuko byoroshye kwanduzwa na bagiteri, kandi igomba gutegurwa mbere yo kuyikoresha. Ugereranije na PAGE tekinike ya electrophoreis, imikorere ya molekile ya molekile ni mike, kandi akarere ka electrophoreis ni gake.
UhagaritseeYamazakisisitemu ya agarose gel
Usibye agarose na buffer, igeragezwa rya agarose gel electrophoreis ikenera sisitemu ya electrophoreis ya horizontal na hasi-voltage electrophoreis itanga amashanyarazi.Beijing Liuyi Biotechnology irashobora gutanga sisitemu ya horizontal electrophoreis kandi amashanyarazi ya electrophoreis, nkicyitegererezoDYCP-31BN, DYCP-31CN Kurisisitemu ya horizontal electrophoreis naDYY-6C yo gutanga amashanyarazi.
Ukurikije ku bunini butandukanye bwa gelbisabwa na agrose gel electrophoreis igeragezwa, ubunini butandukanye bwa sisitemu ya electrophoreis ya horizontal irashobora gutangwa na Beijing Liuyi Biotechnology. Icyitegererezo DYCP-31 ikurikirana,such asDYCP-31BN, DYCP-31CN, DYCP-31DN, DYCP-31E, DYCP-32B, naDYCP-32Cufite ubunini butandukanye bwa gel tray, kuva guta mini nini ya gel kugeza kuri gel nini nini, barashobora guhura nubushakashatsi bwaweibisabwa.
Ikirangantego cya Liuyi gifite amateka arenga imyaka 50 mubushinwa kandi isosiyete irashobora gutanga ibicuruzwa bihamye kandi byujuje ubuziranenge kwisi yose.Mu myaka yiterambere, birakwiye ko uhitamo!
Kubindi bisobanuro kuri twe, nyamuneka twandikire ukoresheje imeri[imeri irinzwe], kugurisha01@ ly.com.cn.
Igihe cyo kohereza: Apr-16-2022