Nigute Gukora ADN Electrophoresis muri Agarose Gel？

Hano tuzasobanura uburyo bwo gukora agarose gel electrophorei numushakashatsi wacu muri laboratoire ya Liuyi Biotech.

Mbere yubushakashatsi, dukeneye kugenzura ibikoresho, reagent, nibindi bikoresho byubushakashatsi nibikoresho dukeneye.

Gutegura ibikoresho byubushakashatsi nibikoresho

Ibikoresho bya agarose gel electrophorei

Horizontal electrophoreis selile (tank / chamber), hagati na voltage yo hasi ya electrophoreis amashanyarazi, gel ishusho & sisitemu yo gusesengura.

Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) itanga imiterere itandukanye ya selile ya electrophoreis ya horizontal (tank / chamber) hamwe nogutanga amashanyarazi hamwe na sisitemu ya gel. Icyitegererezo cyibicuruzwa bya DYCP-31 ni ibya electrophorei, naho ibicuruzwa bya DYY nibyo bitanga amashanyarazi. Ibicuruzwa bya WD-9413 ni sisitemu ya gel. Kuva mubunini bwa gel 60 × 60mm kugeza kuri gel nini 250 × 250mm, turashobora kuzuza ibisabwa bitandukanye kubunini bwa gel. IcyitegererezoDYCP-32Cirashobora gukora gel kugirango igere kuri 250 × 250mm. UwitekaDYY-6Cni amashanyarazi. Ifasha umusaruro wa 400V, 400mA, 240W, nigicuruzwa rusange gikoreshwa nabakiriya bacu. WD-9413BGel Ishusho & Isesengura Sisitemu ikoreshwa mugusesengura no gukora ubushakashatsi kuri gel, firime, na blots nyuma yubushakashatsi bwa electrophoreis. Nigikoresho cyibanze gifite ultraviolet yumucyo wo kwiyumvisha no gufotora gele irangi irangi rya fluorescent nka etidium bromide, hamwe numucyo wera wo gushushanya no gufotora gele yanditswemo amarangi nka coomassie nziza yubururu.

Reagents ya agarose gel electrophorei

1.Uburyo: agarose gel

2.Bffer: TAE (Tris-acetate, EDTA, acide glacial acetique) na TBE (Tris-borate, EDTA, aside boric).

3.Ibikoresho byo gupakira: 6 × Buffer Yipakurura ADN (Bidasanzwe kuri sample ya ADN: EDAT, glycerine, Xylene cyanol na bromophenol ubururu)

Irangi

Irangi rya fluorescent nka EB, Gelred, kureba zahabu, GenGreen, GenView, SYBRGreen

UwitekaIbikoreshwa

Inama ya Sterilisation (10μL), inama ya pipette (200μL), inama ya pipeti (1000μL), 200μL \ 500μL \ 1.5ml ya centrifuge.

Ikimenyetso cya ADN

Byateguwe ukurikije uburemere bwa molekile kugirango bipimwe.

Gel yiruka kandi urebe

Ubwa mbere, dukeneye gutegura gel ya agarose. Ubwinshi bwa agarose muri gel bizaterwa nubunini bwibice bya ADN bigomba gutandukana, hamwe na geles nyinshi ziri hagati ya 0.5% -2%. Gufata ibyacuDYCP-31DNkurugero, Urashobora guta gel nto, gel yagutse, gel ndende na gel kare ukurikije ibisabwa mubushakashatsi. Hitamo tray ya gel ukeneye, hanyuma ushyiremo ibimamara, hanyuma Suka gel ashyushye ya agarose gel mubikoresho bya gel.

Noneho, nyuma ya gele imaze gukomera, fata ibimamara witonze kandi byoroshye, hanyuma ushyire tray ya gel muri tanker. Suka igisubizo cya buffer muri tanker hanyuma ukore gel yose yibizwa muri buffer. Shira ingero mu mariba hamwe na pipeti isanzwe. Huza iDYCP-31DNhamwe na electrophoreis itanga amashanyarazi neza, hanyuma ushireho ibipimo byo gukora gel.

Tekereza ibice bya ADN muri UV transilluminator.

After ikoresha gel, urashobora gukoresha ishusho yacu ya gel & isesengura rya sisitemuWD-9413Bkwitegereza, gusesengura no gufata amashusho ya gel. Liuyi Biotech itanga kandi UV transilluminator (UV Analyser) kugirango yitegereze gel. Dufite umukara-agasanduku ubwoko bwa UV transilluminator (UV Analyser) icyitegererezoWD-9403A, 9403C, WD-9403F, portable UV transilluminator (UV Analyser) icyitegererezoWD-9403Bno gufata UV transilluminator (UV Analyser)WD-9403Ekuko wahisemo.

Ishusho ya gel post electrophorei

Ikirango cya Liuyi gifite amateka yimyaka irenga 50 mubushinwa kandi isosiyete irashobora gutanga ibicuruzwa bihamye kandi byujuje ubuziranenge kwisi yose. Binyuze mumyaka yiterambere, birakwiye guhitamo!

Kubindi bisobanuro kuri twe, nyamuneka twandikire ukoresheje imeri[imeri irinzwe], [imeri irinzwe].