Intangiriro
Gel electrophorei ni tekinike yibanze muri biologiya ya molekuline, ikoreshwa cyane mugutandukanya poroteyine, acide nucleique, nizindi macromolecules. Kugenzura neza urugero rwicyitegererezo, voltage, nigihe cya electrophoreis ningirakamaro kugirango tugere kubisubizo nyabyo kandi byororoka.Mugenzi wa laboratoire aratangauburyo bwiza bwo gucunga ibipimo mugihe SDS-PAGE gel electrophorei.
Beijing Liuyi Biotechnology gel electrophoresis ibicuruzwa
Urugero rw'icyitegererezo: Kwemeza ko bihoraho
Mugihe ukora SDS-PAGE electrophoreis, ingano yicyitegererezo nikintu cyingenzi gishobora kugira ingaruka zikomeye kumyanzuro y'ibisubizo byawe. Mubisanzwe birasabwa gupakira 10 µL ya proteine zose kuriba. Kugirango uhamye kandi wirinde gukwirakwiza icyitegererezo hagati y'iriba ryegeranye, ni ngombwa gupakira ingano ingana na 1x yipakurura buffer mu mariba yose arimo ubusa. Uku kwirinda birafasha gukumira ikwirakwizwa ryintangarugero mumihanda ituranye, bishobora kubaho mugihe iriba risigaye ubusa.
Mbere yo gupakira ingero zawe, burigihe utangire wongereho marike yuburemere kuri iriba. Ibi bituma habaho kumenya byoroshye ingano ya poroteyine nyuma ya electrophorei.
Kugenzura Umuvuduko: Kuringaniza Umuvuduko no Gukemura
Umuvuduko ukoreshwa mugihe cya electrophoreis uhindura muburyo bwihuse umuvuduko ingero zimukira muri gel hamwe no gukemura gutandukana. Kuri SDS-PAGE, nibyiza gutangirana na voltage ntoya ya 80V. Iyi voltage yambere ituma ingero zigenda buhoro kandi buringaniye, zikabishyira mumurongo utyaye uko zinjiye muri gel itandukanya.
Ingero zimaze kwinjira neza muri gel itandukanya, voltage irashobora kwiyongera kuri 120V. Umuvuduko mwinshi wihuta kwimuka, ukemeza ko poroteyine zitandukanijwe neza ukurikije uburemere bwa molekile. Ni ngombwa gukurikirana imigendekere yimbere ya bromophenol yubururu, byerekana irangizwa rya electrophore. Kuri geles hamwe na 10-12%, iminota 80-90 mubisanzwe birahagije; icyakora, kuri 15% geles, urashobora gukenera kongera igihe cyo kwiruka gato.
Gucunga Igihe: Kumenya Igihe cyo Guhagarara
Igihe nikindi kintu gikomeye muri gel electrophorei. Gukoresha gel igihe kirekire cyane cyangwa kigufi cyane mugihe gishobora gutuma umuntu atandukana. Kwimuka kwa bromophenol irangi ry'ubururu ni ikimenyetso cyingirakamaro: iyo kigeze munsi ya gel, mubisanzwe ni igihe cyo guhagarika kwiruka. Kuri gele isanzwe, nka 10-12%, electrophoreis yamara iminota 80-90 mubisanzwe irahagije. Kuri jel ijanisha ryinshi, nka 15%, igihe cyo gukora kigomba kongerwa kugirango habeho gutandukana kwuzuye kwa poroteyine.
Ubuyobozi bwa Buffer: Gukoresha no Gutegura Buffers
Buffer ya Electrophoresis irashobora gukoreshwa inshuro 1-2, bitewe na laboratoire yawe yihariye. Ariko, kubisubizo byiza, birasabwa gutegura buffer 10x hanyuma ukayungurura mbere yo kuyikoresha. Ibi byemeza ko buffer ikomeza gukora neza, biganisha kubisubizo bya electrophoreis byizewe.
Beijing Liuyi Biotechnology gel electrophoresis ibicuruzwa
Mugenzuye neza urugero rwicyitegererezo, voltage, hamwe nigihe cya electrophoreis, urashobora kunonosora neza ukuri no kubyara ibisubizo bya gel electrophorei. Gushyira mubikorwa ibyo byiza mubikorwa bya laboratoire bizagufasha kugera kumirongo isobanutse kandi itandukanye, biganisha kumakuru meza yo gusesengura hasi.
Niba ufite uburyo bwiza bwo kunoza igeragezwa rya gel electrophorei, urakaza neza kugirango tuganire natwe!
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ifite ubuhanga mu gukora ibikoresho bya electrophoreis mu myaka irenga 50 hamwe nitsinda ryacu ryubuhanga ryumwuga hamwe nikigo cya R&D. Dufite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe ninkunga yo kwamamaza. Ibicuruzwa byacu byingenzi ni Akagari ka Electrophoresis (tank / chamber), Electrophoresis Amashanyarazi, Ubururu LED Transilluminator, UV Transilluminator, Gel Image & Analyse Sisitemu nibindi kandi dutanga ibikoresho bya laboratoire nkibikoresho bya PCR, mixer ya vortex na centrifuge ya laboratoire.
Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.
Nyamuneka Sikana kode ya QR kugirango wongere kuri Whatsapp cyangwa WeChat.
Igihe cyo kohereza: Kanama-20-2024