Imyitwarire ya Polymerase (PCR) na Gel Electrophoresis: Ubuhanga Bwingenzi muri Biologiya ya Molecular

Mubice bigenda byiyongera mubinyabuzima bya molekuline, Polymerase Chain Reaction (PCR) na Gel Electrophoresis byagaragaye nkubuhanga bwibanze bworohereza ubushakashatsi no gukoresha ADN. Ubu buryo ntabwo bukoreshwa mubushakashatsi gusa ahubwo bufite nuburyo bukoreshwa mugusuzuma, siyanse yubucamanza, na biotechnologiya.

Ishusho kuva kurubuga biologiya4alevel

PCR nubuhanga bwimpinduramatwara bwakozwe na Kary Mullis mu 1983, butuma abahanga bongerera igice ADN igice cyihariye. Inzira itangirana no gutandukanya ADN, aho ADN ifite imirongo ibiri ishyutswe kugeza kuri 94 ° C, bigatuma itandukana mumirongo ibiri imwe. Ibyo bikurikirwa no gufatira hamwe, aho primers - urukurikirane rugufi rwa nucleotide - ihuza urwego rwuzuzanya kuri ADN imwe rukumbi ku bushyuhe buke (ubusanzwe hafi 55 ° C). Hanyuma, icyiciro cyo kwaguka kibaho kuri 72 ° C, aho enzyme ADN polymerase ihuza umurongo mushya wa ADN wongeyeho nucleotide kuri primers. Uru ruzinduko rusubirwamo inshuro 20-40, biganisha kuri miriyoni za kopi yintego za ADN zikurikirana.

Beijing LIUYI Imashini ya PCR

ADN imaze kwongerwa, Gel Electrophoresis ikoreshwa mugutandukanya no gusesengura ibicuruzwa bya PCR. Ubu buhanga bukubiyemo kwimuka kwa ADN binyuze muri materique ya agarose yatewe n'umuriro w'amashanyarazi. Molekile ya ADN yishyurwa nabi kubera umugongo wa fosifate, kandi bimuka bigana kuri electrode nziza. Gele ikora nk'icyuma, ituma uduce duto twa ADN tugenda vuba kurusha nini. Kubera iyo mpamvu, ibice bya ADN bitandukanijwe bitewe nubunini bwabyo, hamwe nimirongo itandukanye igaragara munsi yumucyo ultraviolet nyuma yo kuyisiga irangi nka bromide ya Ethidium.

Beijing LIUYIGel Ibicuruzwa bya Electrophoresis

Ihuriro rya PCR na Gel Electrophoresis irakomeye mubikorwa byinshi. Mu gusuzuma indwara, PCR ikoreshwa mu kumenya ko hari indwara ziterwa na virusi, ihinduka ry’imiterere, cyangwa ADN zikurikirana zerekeye indwara. Gel Electrophoresis noneho yemerera kubona no kwemeza ibyo bice bya ADN byongerewe. Muri siyansi yubucamanza, ubwo buhanga ni ingenzi cyane mu gutunga urutoki rwa ADN, aho bifasha guhuza ingero za ADN zerekanwe aho ibyaha byakorewe n’abakekwaho icyaha.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ifite ubuhanga mu gukora ibikoresho bya electrophoreis mu myaka irenga 50 hamwe nitsinda ryacu ryubuhanga ryumwuga hamwe nikigo cya R&D. Dufite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe ninkunga yo kwamamaza. Ibicuruzwa byacu byingenzi ni Akagari ka Electrophoresis (tank / chamber), Electrophoresis Amashanyarazi, Ubururu LED Transilluminator, UV Transilluminator, Gel Image & Analyse Sisitemu nibindi kandi dutanga ibikoresho bya laboratoire nkibikoresho bya PCR, mixer ya vortex na centrifuge ya laboratoire.

Beijing LIUYIGel Ibicuruzwa bya Electrophoresis

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.

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