Gutegura Agarose Gel Kuri Electrophoresis

Gutegura Agarose Gel kuri Electrophoresis

Icyitonderwa: Buri gihe wambare uturindantoki twajugunywe!

Intambwe ku yindi

Gupima ifu ya Agarose:use gupima impapuro nuburinganire bwa elegitoronike gupima 0.3g yifu ya agarose (ishingiye kuri sisitemu ya 30ml).

Gutegura Buffer ya TBST:psubiza 30ml ya 1x TBST ya buffer muri flask ya 100ml Erlenmeyer.

Kumenagura ifu ya Agarose:pifu ya agarose muri buffer ya TBST hanyuma unyeganyeze neza.Shyiramokuri microwave nubushyuhe (mubisanzwe kumasegonda 50, bitwikiriwe na aluminiyumu) ​​kugeza bishonge burundu.

Gukonjesha no Kongera Nuclease:use gants kugirango ukureho imvange muri microwave hanyuma ureke bikonje gato mumazi akonje kugeza ashyushye (hafi 60 ° C). Ongeramo 2µl ya nuclease (Eb isimbuye) mugihe uhinda umushyitsi kugirango uvange neza.

Gutegura Gel Mold:

• CKunama no gukamagel tray hamwe na gel castingya tank ya electrophoreis.
•  Place geltraymu kigega cy'imbere hanyuma ushyiremo ibimamara ahantu hateganijwe.
• Kuvanga umuti wa agarose, ukonje kugeza kuri 65 ° C, hanyuma ubisuke witonze kurigel trayinibikoresho bya gel, kuyikwirakwiza gahoro gahoro kugeza igize na gel igaragara ku isahani yikirahure.
• Emerera kwicara ku bushyuhe bwicyumba kugeza gel ikomera rwose.
• Kuramo buhoro buhoro ikimamara uhagarike kandi ukureho kaseti.
• Shira geltraymuri tank ya electrophoreis.

Icyangombwa: Menya neza ko nta mwuka uhumeka uri mu menyo yikimamara. Ubuso bwamazi bugomba kuba bworoshye nta guhindagurika.

Gukoresha Gel

Gutwara Gel

Gele imaze gukomera, shyira muri tank ya electrophoreis hanyuma usukemo buffer ya electrophoreis kugeza igihe geli irohamye.

Gutegura Ingero

• Fata marikeri no gupakira buffer muri firigo.
• Ongeramo 6µl yo gupakira buffer kurugero hanyuma uvange neza.
• Ukoresheje micropipette, shyira buhoro buhoro ingero mumariba manini ya gel (witondere kudacumita gele kandi ntutange amajwi yose kugirango wirinde umwuka mubi).
• Shira akamenyetso muri buri riba rito (ibuka umwanya waryo).

Gutangira Electrophoresis

• Gupfuka ikigega cya electrophoreis hanyuma utangire electrophoreis ukimara gupakira gel.
• Shyira voltage kuri 60-100V. Ibyitegererezo bizimuka biva kuri electrode mbi (umukara) kuri electrode nziza (umutuku).
• Umuvuduko mwinshi ugabanya itandukaniro ryiza rya agarose gel.
• Hagarika electrophoreis mugihe irangi ry'ubururu rya bromophenol rigeze kuri 1cm uhereye kumpera yo hepfo ya plaque.

Kureba ibisubizo

Nyuma yo gutandukanya bande, hagarika electrophoreis, fata gel, hanyuma umenye neza kandi uyifotore.

Koresha uburyo bwo gufata amashusho ya gel kugirango ufotore kandi witegereze bande (reba niba hari bande ya marikeri cyangwa ingero).

Nyuma yo kubona ikarita yawe ya gel, shakisha akamenyetso. Ukurikije marikeri, urashobora kumenya intego zitsinda!

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) imaze imyaka irenga 50 ikorerwa ibicuruzwa bya electrophoreis. Turerekana sisitemu ya horizontal electrophoreis sisitemu yaagarose gelamashanyarazi.Hitamosisitemu ya horizontal electrophoreiskuva i Beijing liuyi kubigeragezo bya gel electrophoresis kugirango bigufashe ubushakashatsi bwawe mubukorikori bwibinyabuzima.

Dutanga moderi zitandukanye za horizontal electrophoreis tanks yo guta no gukora ntoagarosegeles to bigagarosegeles

Guhitamo Ubuyobozi bwa Horizontal Immers Gel Gel Electrophoresis Igice

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ifite ubuhanga mu gukora ibikoresho bya electrophoreis mu myaka irenga 50 hamwe nitsinda ryacu ryubuhanga ryumwuga hamwe nikigo cya R&D. Dufite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe ninkunga yo kwamamaza. Ibicuruzwa byacu byingenzi ni Akagari ka Electrophoresis (tank / chamber), Electrophoresis Amashanyarazi, Ubururu LED Transilluminator, UV Transilluminator, Gel Image & Analyse Sisitemu nibindi kandi dutanga ibikoresho bya laboratoire nkibikoresho bya PCR, mixer ya vortex na centrifuge ya laboratoire.

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.

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