IncamakeByaAgarose Gel Electrophoresis
Agarose gel electrophorei ni tekinike ikoreshwa cyane muri biologiya ya molekuline yo gutandukanya acide nucleique, nka ADN na RNA, ukurikije ubunini bwayo. Ubu buryo bukoresha gel ikozwe muri agarose, polyisikaride isanzwe ikomoka ku nyanja. Gele ikora nk'icyuma cya molekile, ituma molekile zinyuramo binyuze mugihe amashanyarazi akoreshejwe. Ubu buryo bwo gutandukana bufasha abashakashatsi gusesengura no kuranga ingero za acide nucleic bareba imirongo itandukanye ikozwe ukurikije ubunini bwayo. Agarose gel electrophorei nigikoresho cyibanze kumirimo nko gusesengura ibice bya ADN, gusuzuma isuku, hamwe nubushakashatsi bwibinyabuzima bya molekile.
Mubisanzwe, agarose gel electrophoreis yitwa tank ya horizontal electrophoresis nayo. Ibyingenzi byingenzi bya agarose gel electrophorei zirimo:
Isesengura ry'ibice bya ADN:
Gutandukanya no gusesengura ibice bya ADN bishingiye ku bunini, ni ngombwa ku mirimo nka genotyping na ADN yo gutunga urutoki.
Isesengura rya RNA:
Gukemura molekile ya RNA, ifasha mukwiga imiterere ya gene imvugo nubusugire bwa RNA.
Kwemeza ibicuruzwa bya PCR:
Kugenzura ibicuruzwa bya polymerase byerekana (PCR) kugirango bigerweho neza kandi ubone ingano yukuri.
Isesengura rya ADN ya plasma:
Gusuzuma ADN ya plasmid kugirango igenzure ubuziranenge, kugenzura ubushakashatsi bwakozwe na clon, no kumenya ADN ya recombinant.
Isuzuma ryera:
Kumenya ubuziranenge bwa acide nucleique yerekana amashusho ashobora kwanduza cyangwa umwanda.
Ubushakashatsi ku binyabuzima bya molekuline:
Igikoresho cyibanze muri laboratoire ya biologiya ya laboratoire yubushakashatsi butandukanye, harimo imyiteguro ya ADN ikurikirana hamwe nudukoko twa enzyme.
Beijing Liuyi Biotechnology Co., Ltd. (yahoze ari Uruganda rw’ibikoresho bya Beijing Liuyi) yashinzwe mu 1970. Ni uruganda rw’ikoranabuhanga ruzobereye mu gukora ibinyabuzima byifashishwa mu gusesengura ibinyabuzima na biologiya biologiya.Liuyi imaze imyaka isaga 50 ikora ibikoresho bya elegitoroniki. , kandi ifite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe nubufasha bwo kwamamaza. Ibicuruzwa byingenzi ni Akagari ka Electrophoresis (tank / chamber), Amashanyarazi ya Electrophoresis, Ubururu bwa LED Transilluminator, UV Transilluminator, Gel Image & Analyse nibindi.
Tumenyeshe byinshi kubijyanye na moderi imwe ya agarose gel electrophorei-DYCP-31DN.
Intangiriro yagarose gel electrophoreitank DYCP-31DN
Igipimo (LxWxH) | 310 × 150 × 120mm |
Ingano ya Gel (LxW) | 60 × 60mm, 60 × 120mm, 120 × 60mm, 120 × 120mm |
Comb | Iriba 2 + 3, iriba 6 + 3, iriba 8 + 18, n'iriba 11 + 25 |
Kurwanya Ubunini | 1.0mm, 1.5mm na 2.0mm |
Umubare w'icyitegererezo | 2-100 |
Umubumbe wa Buffer | 650ml |
Ibiro | 1.0kg |
DYCP-31DN ni ya Liuyi ya agarose gel electrophoreis, hamwe nigikoresho cyo guteramo kiboneka cyo guta ibice 1-4. Irashobora gukora jel ntoya ebyiri zingana na 60x60mm na gel imwe yagutse 120x60mm cyangwa gel ndende 60x120mm icyarimwe. Gele nini ishobora gutera ni ubunini bwa 120x120cm.
Uwitekaubunini bwikimamara butangwani1.0mm, 1.5mm na 2.0mm. Hamwe nigishushanyo cya electrode ikurwaho, biroroshye cyane gusukura no kubungabunga. "Igishushanyo mbonera" kigamije kubungabunga igisubizo cya buffer mugihe cya electrophoreis. Gukoresha neza bufferi ningirakamaro mugukomeza amashanyarazi ahamye no kwemeza gutandukanya molekile neza.
Ibindi bizaza by'ingenzi:
1.
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3) Iriba ntangarugero akenshi biragoye kubona, umurongo wumukara rero kuri gel tray bituma byoroha kwitegereza no gupakira ingero.
4) Imbaraga zirananirana mugihe ufunguye umupfundikizo wumutekano wumukoresha.
Hariho uburyo bwinshi bwo gutanga amashanyarazi atangwa kuri DYCP-31DN. Amashanyarazi ashyushye cyane amashanyarazi is DYY-6C. Murakaza neza gusura urubuga rwuburyo bwinshi bwo gutanga amashanyarazi.
Kubona amashusho tweagarose gel hamweADN cyangwa RNA, Liuyi itanga UV transilluminator kubakiriya.WD-9403C, WD-9403B naWD-9403X byose birahari kubakiriya guhitamo.
Agarose gel electrophorei ni ingirakamaro kumirimo nko kugenzura ingano y'ibice bya ADN, gusuzuma ubuziranenge bw'icyitegererezo cya ADN, cyangwa gutandukanya ibice bitandukanye bya ADN kugirango bisesengure.
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Igihe cyo kohereza: Mutarama-11-2024