Poroteyine Electrophoresis Ibibazo Bisanzwe (2)

Twasangiye ibibazo bimwe bisanzwe bijyanye na bande ya electrophoreis mbere, natwe'd nkunda gusangira ibindi bintu bidasanzwe bya polyacrylamide gel electrophorei kurundi ruhande. Twebweincamake ibi bibazo kubakiriya bacu' kwifashisha kugirango umenye impamvu nakubona ibisubizo nyabyo no kunoza ireme rya protein.

Gel ntabwo polymerized Birashoboka

Impamvu ni:

(a) Ubuziranenge bwa monomer budahagije, busaba kongera gukora.

(b) Umwuka wa ogisijeni ushonga mu gisubizo cya gel urabuza polymerisiyumu. Pompe ikora neza igomba gukoreshwa.

(c) Ammonium persulfate ntigikora cyangwa idahagije mubwinshi. Tegura neza cyangwa ukoreshe ikindi gice cya amonium perulfate hamwe nibitekerezo byinshi.

 Tgel yamaze gukora polymerize atongeyeho amazi.

Impamvu ni:

(a) Hisha igisubizo cya gel mbere yo kongeramo catalizator kugirango ugabanye igipimo cya polymerisation.

(b) Kugabanya ingano ya TEMED cyangwa ammonium persulfate yakoreshejwe.

(c) Kwihutisha ibikorwa.

Gel iranyerera mu cyumba cya electrophoreis nyuma ya polymerisation

Ibi bikunze kugaragara cyane muri geles nkeya. Igisubizo kimwe ni ugupfunyika dialyse munsi yigituba cyangwa ugakoresha polipropilene.

Icyitegererezo nticyagaragaye nyuma ya electrophoreis

Impamvu ni:

(a) Umubare w'icyitegererezo udahagije. Ongera umubare wicyitegererezo.

(b) Kwanduza ibintu byakemuwe cyangwa kwibanda cyangwa igihe cyo gusiga ntibihagije. Simbuza igisubizo cyanduye kandi wongere ibitekerezo hamwe nigihe cyo gusiga.

(c) Icyitegererezo cyareremba mugihe cyo gupakira. Ongera ubucucike bwicyitegererezo kandi ukore witonze.

(d) Gutandukanya gel yibanze cyane, kandi sample ntabwo yinjiye muri gel. Hindura icyerekezo cya gel gikwiye.

. Hindura imiterere ya gel hamwe nuburyo bwiza bwa electrophoreis.

(f) Niba icyitegererezo ari RNA, irashobora kuba irimo proteyine zigize urwego runini ruhagarika imyenge ya gel. Kuraho neza poroteyine kurugero rwa RNA.

(g) Icyitegererezo kirimo imisemburo itanga hydrolyze icyitegererezo, kandi icyitegererezo cyangirika mugihe cya electrophorei. Sukura neza icyitegererezo.

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Beijing Liuyi Biotechnology Company Ltd ikora ibicuruzwa bitandukanye bya electrophoreis bishobora gufasha gukemura ibibazo wenda duhura nabyo mubushakashatsi bwa electrophoreis. Isosiyete yashinzwe mu 1970.Yari iy'igihugu kandi ikora imashini yo gusudira amashanyarazi na metero zitembera mu nganda muri kiriya gihe. Kuva mu 1979, Beijing Liuyi Biotechnology Company Ltd itangira gukora ibicuruzwa bya electrophoreis. Ubu isosiyeteis umwe mu bayoborauwakoze ibikoresho bya laboratoire nibikoresho bya siyansi bishingiye i Beijing, mu Bushinwa. Yayo ikirangoLIUYI azwi cyane mu Bushinwa muri kano karere.

Ibicuruzwa by'isosiyete birimo ibikoresho byinshi bya laboratoire, harimo ikigega cya nucleic acide electrophoreis tank, vertical protein electrophoresis tank / unit, umukara-agasandukuUbwoko UV isesengura,Gel Inyandiko Ikurikirana Imashusho Yisesengura, hamwe na electrophoreis itanga amashanyarazi. Ibicuruzwa bikoreshwa cyane mubigo byubushakashatsi, kaminuza, ninganda zitandukanye, nka farumasi, ibinyabuzima, no gukurikirana ibidukikije.Isosiyete ni ISO9001 & ISO13485 isosiyete yemewe kandi ifite ibyemezo bya CE.

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Harihoubwoko butandukanye bwauhagaritseibigega bya electrophoreisprotein electrophoreisyo gusesengura no kumenya intangarugero za poroteyine na polyacrylamide gel electrophorei,nano gupima uburemere bwa molekuline uburemere, kweza ingero no gutegura ingero.Ibicuruzwa byose biremewe muriisoko ryo mu gihugu no hanze.

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Amashanyarazi ya electrophoreis nikintu cyingenzi cyasisitemu ya electrophoreis, gutanga isoko ihamye kandi yuzuye yumuriro wamashanyarazi kugirango utware inzira yo gutandukana.Itmubisanzwe itanga voltage ihoraho cyangwa ihoraho kuri sisitemu ya electrophoreis, bitewe na protocole yihariye yubushakashatsi. Iyemerera kandi uyikoresha guhindura voltage cyangwa ibisohoka muri iki gihe, kimwe nibindi bipimo nkigihe nubushyuhe, kugirango ahindure imiterere yo gutandukana kubushakashatsi runaka.

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Fcyangwa kwitegereza gel, urashobora guhitamo UV Transilluminator WD-9403 yakozwe na Beijing Liuyi Biotechnology.A UV transilluminator nigikoresho cya laboratoire ikoreshwa mugushushanya no gusesengura ADN, RNA, na proteine. Ikora mu kumurika ibyitegererezo hamwe nurumuri rwa UV, itera ingero za fluoresce kandi zigaragara. Hariho moderi nyinshi za UV transilluminatoryatanzwe nawe. WD-9403A ni umwihariko wo kureba poroteyine electrophorei, kandi WD-9403F ikoreshwa mu kureba ADN na poroteyine electrophorei.

Uru ruhererekane rw'ibicuruzwa rushobora gutanga kuva kuri casting kugeza kwitegereza gel ukurikije ibyo usabwa gukora.Tumenyeshe ibyo usabwa, OEM, ODM hamwe nababigenewe murakaza neza.We tuzagerageza uko dushoboye kugirango tuguhe ibicuruzwa na serivisi.

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.


Igihe cyo kohereza: Gicurasi-23-2023