Intambwe-ku-ntambwe yo kuyobora Gutegura Agarose Gel kuri Electrophoresis

Ufite ingorane zo gutegura gel agarose?Reka dukurikiraneumutekinisiye wa laboratoire mugutegura gel.

Gahunda yo gutegura agarose gel ikubiyemo intambwe zikurikira:

Gupima ifu ya Agarose

Gupima ingano isabwa ya poro ya agarose ukurikije icyifuzo cyawe kugirango ugerageze. Ubusanzwe agarose yibanda kuri 0.5% kugeza 3%. Ubunini bwinshi bukoreshwa mugutandukanya molekile ntoya ya ADN, mugihe intumbero yo hasi ari ya molekile nini.

Gutegura igisubizo cya Buffer

Ongeramo ifu ya agarose kuri buffer ya electrophoreis ikwiye, nka 1 × TAE cyangwa 1 × TBE. Ingano ya buffer igomba guhuza ingano ya gel ikenewe mubushakashatsi bwawe.

Gusenya Agarose

Shyira agarose hamwe na buffer ivanze kugeza agarose ishonga burundu. Ibi birashobora gukorwa ukoresheje microwave cyangwa isahani ishyushye. Kangura igisubizo rimwe na rimwe kugirango wirinde guteka. Igisubizo cya agarose kigomba gusobanuka nta kintu kigaragara.

Gukonjesha Agarose

Emera igisubizo gishyushye cya agarose gukonja kugeza kuri 50-60 ° C. Koresha igisubizo mugihe cyo gukonjesha kugirango wirinde gukomera hakiri kare.

Ongeramo Nucleic Acide Ikirangantego (Bihitamo)

Niba ushaka kwiyumvisha ADN cyangwa RNA muri gel, urashobora kongeramo aside nucleic aside, nka GelRed cyangwa etidium bromide, muriki cyiciro. Mugihe ukoresha ayo mabara, ambara uturindantoki kandi witondere, kuko bishobora kuba uburozi.

Gutera Gel

Suka igisubizo gikonje cya agarose muburyo bwa electrophoreis gel. Shyiramo ibimamara kugirango ukore icyitegererezo cy'iriba, urebe ko ikimamara gifite umutekano kandi igisubizo kigabanijwe neza.

 Gukomera kwa Gel

Emerera gele gukomera mubushyuhe bwicyumba, mubisanzwe bifata iminota 20-30 bitewe nubunini nubunini bwa gel.

RKwimura Ikimamara

Gele imaze gukomera rwose, kura neza ikimamara kugirango ugaragaze amariba y'icyitegererezo. Shira gele hamwe nububiko mucyumba cya electrophoreis hanyuma uyipfundikire hamwe na bffer ya electrophoresis ikwiye, urebe ko gel yuzuye.

Gutegura amashanyarazi

Gele imaze kwitegura, shyira ingero zawe mu mariba, hanyuma ukomeze ubushakashatsi bwa electrophoreis.

Niba ufite ikibazo cyo gutegura gel, wumve neza. Dufite abatekinisiye babigize umwuga baboneka kugirango tuvugane nawe.

Tunejejwe no kubagezaho amakuru akomeye: ikigega cyacu DYCP-31DN kizwi cyane cya horizontal electrophoreis iri kuri promotion, twandikire kubindi bisobanuro nonaha!

DYCP-31DN itambitse ya horizontal electrophoreis

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ifite ubuhanga mu gukora ibikoresho bya electrophoreis mu myaka irenga 50 hamwe nitsinda ryacu ryubuhanga ryumwuga hamwe nikigo cya R&D. Dufite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe ninkunga yo kwamamaza. Ibicuruzwa byacu byingenzi ni Akagari ka Electrophoresis (tank / chamber), Electrophoresis Amashanyarazi, Ubururu LED Transilluminator, UV Transilluminator, Gel Image & Analyse Sisitemu nibindi kandi dutanga ibikoresho bya laboratoire nkibikoresho bya PCR, mixer ya vortex na centrifuge ya laboratoire.

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.

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