Intambwe Zibikorwa bya Semi-yumye Trans Blot Ibikoresho DYCP-40C

Sisitemu ya DYCP-40C yumye yumye ikoreshwa hamwe nogutanga amashanyarazi ya electrophoreis kugirango yohereze poroteyine muri geles ya polyacrylamide kuri membrane nka nitrocellulose membrane, nylon membrane na membrane ya PVDF. Semi-yumye ikorwa hamwe na grafite ya plaque electrode muburyo butambitse, sandwiching gel hamwe na membrane hagati yimpapuro zimpapuro zometse kumpapuro zungurura zikora nkikigega cya ion. Mugihe cyo guhererekanya amashanyarazi, molekile zashizwemo nabi ziva muri gel zikagenda zerekeza kuri electrode nziza, aho zishyirwa kuri membrane. Isahani ya electrode, itandukanijwe gusa na gel hamwe no kuyungurura impapuro, itanga imbaraga zo murwego rwo hejuru (V / cm) hejuru ya gel, ikora ihererekanyabubasha ryiza cyane. Ihererekanyabubasha rya DYCP ntoya - 40C selile electrophoreis ni 150 × 150 (mm), ibereye kwimura geles zisanzwe, harimo iziva muri DYCZ-24DN na DYCZ-24EN selile electrophoreis.

Reka twige byinshi kubyerekeye imikorere yiki gice cyumye cyumye.

Ibikoresho, ibikoresho byo gukora DYCP-40C
Amashanyarazi ya Electrophoresis DYY-6C, ibikoresho byumye byumye byumye DYCP-40C, igisubizo cya buffer, hamwe nibikoresho byo gukemura. n'ibindi

Intambwe zo Gukora
1. Shyira gel hamwe nibisahani mubirahure byoherejwe

2. Gupima ubunini bwa gel

3.Tegura ibice 3 byimpapuro zungurura ukurikije ubunini bwa gel, kandi ubunini bwimpapuro zungurura bigomba kuba binini gato kurenza ubunini bwa gel; Hano dukoresha urupapuro rwa filteri ya Whatman;

4.Shira ibice 3 byimpapuro muyungurura buhoro buhoro, hanyuma ureke impapuro ziyungurura zishire muri buffer rwose, kandi wirinde kugira umwuka mwinshi;

5.Gutegura no gukata membrane ya nitrocellulose ukurikije ubunini bwa gel hamwe nimpapuro; ingano ya nitrocellulose membrane igomba kuba nini kuruta ubunini bwa gel hamwe nimpapuro;

6.Shira nitrocellulose membrane mubisubizo bya buffer;

7.Kuramo ibice 3 byungurura impapuro hanyuma uta ibisubizo byongeweho kugeza igihe nta gisubizo cya buffer kiva muri membrane; hanyuma ushireho akayunguruzo munsi ya DYCP-40C;

8.Fata gel mu isahani y'ibirahure, usukure witonze geli yegeranye, hanyuma ushyire gel mubisubizo bya buffer;

9.Shira gele kumpapuro ziyungurura, tangira uhereye kumpera imwe ya gel kugirango wirinde umwuka mubi;

10.Koresha igikoresho gikwiye kugirango ukureho umwuka mwinshi hagati ya gel nimpapuro.

11.Gupfuka membrane ya nitrocellulose kuri gel, uruhande rutoroshye rugana kuri gel. Noneho koresha igikoresho gikwiye kugirango ukureho umwuka mwinshi hagati ya membrane na gel. Shira ibice 3 byiyungurura impapuro kuri membrane. Biracyakenewe gukoresha igikoresho gikwiye kugirango ukureho umwuka mwinshi hagati yimpapuro zungurura na membrane.

12.Gupfundikira umupfundikizo, hanyuma ushireho electrophoreis ikora ibipimo, 80mA ihoraho;

13.Electrophoresis irakorwa. Twabonye ibisubizo nkibi bikurikira;

Beijing Liuyi Biotechnology Co., Ltd imaze imyaka irenga 50 ikora ibijyanye no gukora ibicuruzwa bya electrophoreis. Turi ISO9001 & ISO13485 isosiyete yemewe kandi ifite ubuhanga bwo gukora ibigega bya electrophoreis, ibikoresho byamashanyarazi, UV transilluminator, hamwe na gel documentaire & sisitemu, hagati aho, dutanga serivisi ya OEM kubakiriya bacu, ndetse na serivisi ya ODM.

Ubu turimo gushakisha abafatanyabikorwa, OEM n'abayitanga barahawe ikaze.

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.