PCR Ubushuhe bw'amagare WD-9402M
Ibisobanuro
| Icyitegererezo | WD-9402M |
| Ubushobozi | 96 × 0.2ml |
| Tube | 96x0.2ml (isahani ya PCR idafite / ijipo ya kabiri), imirongo ya 12x8x0.2ml, imirongo ya 8x12x0.2ml, imiyoboro 0.2ml (uburebure bwa 20 ~ 23mm) |
| Hagarika Ubushyuhe | 0-105 ℃ |
| Hagarika Ubushyuhe Bwuzuye | ± 0.2 ℃ |
| Hagarika Ubushyuhe Bumwe | ± 0.5 ℃ |
| Gushyushya Igipimo (ugereranije) | 4 ℃ |
| Gukonjesha Igipimo (ugereranije) | 3 ℃ |
| Kugenzura Ubushyuhe | Guhagarika / Tube |
| Gradient Temp. Urwego | 30-105 ℃ |
| Icyiciro | 5 ℃ / s |
| Igipimo. Igipimo cyo gukonjesha 4.5 ℃ / S. | 4.5 ℃ / s |
| Gradient Gushiraho | Icyiza. 42 ℃ |
| Ubushyuhe bwa Gradient | ± 0.3 ℃ |
| Ubushyuhe bwerekana neza | 0.1 ℃ |
| Gushyushya Umupfundikizo Urwego | 30 ℃ ~ 110 ℃ |
| Gushyushya Igikoresho | Funga mu buryo bwikora mugihe icyitegererezo kiri munsi ya 30 ℃ cyangwa porogaramu irangiye |
| Igihe cyiyongera / Kugabanuka | -599 ~ 599 S kuri PCR ndende |
| Ubushyuhe Kwiyongera / Kugabanuka | -9.9 ~ 9.9 ℃ kuri Touchdown PCR |
| Igihe | 1s ~ 59min59sec / Itagira iherezo |
| Porogaramu zibitswe | 10000+ |
| Amagare | 99 |
| Icyiciro | 30 |
| Kuruhuka Imikorere | Yego |
| Imikorere yo gukoraho | Yego |
| Imikorere miremire ya PCR | Yego |
| Ururimi | Icyongereza |
| Gahunda yo Kuruhuka Imikorere | Yego |
| 16 function Imikorere yo gufata ubushyuhe | Itagira iherezo |
| Imikorere-nyayo | Ishusho-inyandiko yerekanwe |
| Itumanaho | USB 2.0 |
| Ibipimo | 200mm × 300mm × 170mm (W × D × H) |
| Ibiro | 4.5kg |
| Amashanyarazi | 100-240VAC, 50 / 60Hz, 600W |
Ibisobanuro
Amagare yumuriro akora ashyushya inshuro nyinshi no gukonjesha imvange ya reaction irimo ADN cyangwa RNA icyitegererezo, primers, na nucleotide. Ubushyuhe bwo gusiganwa ku magare bugenzurwa neza kugirango bugere ku gutandukana gukenewe, guhuza, no kwagura intambwe za PCR.
Mubisanzwe, umukinnyi wamagare yumuriro afite blok irimo amariba menshi cyangwa imiyoboro aho imvange ya reaction ishyizwe, kandi ubushyuhe muri buri riba bugenzurwa bwigenga. Inzitizi irashyuha kandi ikonjeshwa ukoresheje ikintu cya Peltier cyangwa ubundi buryo bwo gushyushya no gukonjesha.
Amagare menshi yubushyuhe afite umukoresha-wifashisha interineti yemerera uyikoresha gahunda no guhindura ibipimo byamagare, nkubushyuhe bwa annealing, igihe cyo kwagura, numubare wizunguruka. Bashobora kandi kwerekana ibyerekanwe kugirango bakurikirane imigendekere yimyitwarire, kandi moderi zimwe zishobora gutanga ibintu byambere nka progaramu ya progaramu ya progaramu ya progaramu ya buke, ibishushanyo mbonera byinshi, hamwe no kugenzura kure no kugenzura.
Gusaba
Polymerase Chain Reaction (PCR) ni tekinike ya biologiya ikoreshwa cyane mubikorwa bitandukanye. Bimwe mubisanzwe porogaramu ya PCR irimo:
Kwiyongera kwa ADN: Intego yibanze ya PCR ni ukongera ADN ikurikiranye. Ibi nibyingenzi kubona ADN ihagije kugirango isesengure cyangwa igeragezwa.
Kwipimisha genetike: PCR ikoreshwa cyane mugupima geneti kugirango hamenyekane ibimenyetso byihariye bya genetike cyangwa ihinduka ryimiterere yindwara. Nibyingenzi mubikorwa byo gusuzuma no kwiga genetisifique.
Gukwirakwiza ADN: PCR ikoreshwa kugirango habeho igice kinini cya ADN yihariye, ishobora noneho gukoronizwa mubice kugirango ikoreshwe cyangwa isesengura.
Isesengura rya ADN ya Forensic: PCR ningirakamaro muri siyanse yubucamanza kugirango hongerwe umunota ADN yakuwe ahakorewe ibyaha. Ifasha mukumenya abantu no gushiraho isano.
Microbial Detection: PCR ikoreshwa mugutahura mikorobe ziterwa na clinique cyangwa ibidukikije. Iremera kumenyekanisha byihuse imiti yanduye.
PCR ihagije (qPCR cyangwa Real-Time PCR): qPCR itanga umubare wa ADN mugihe cyo kongera imbaraga. Ikoreshwa mugupima imiterere ya gene imvugo, kumenya imitwaro ya virusi, no kugereranya ingano ya ADN ikurikiranye.
Ubushakashatsi bwa Molecular: PCR ikoreshwa mubushakashatsi bwerekana itandukaniro rishingiye ku gitsina mu baturage, isano y'ubwihindurize, hamwe na analyse ya phylogeneque.
Isesengura rya ADN y’ibidukikije (eDNA) Isesengura: PCR ikoreshwa kugirango hamenyekane ko hari ibinyabuzima byihariye mu ngero z’ibidukikije, bigira uruhare mu binyabuzima ndetse n’ubushakashatsi bw’ibidukikije.
Ubwubatsi bwa genetike: PCR nigikoresho cyingenzi mubwubatsi bwa geneti kugirango tumenye ADN ikurikiranye mubinyabuzima. Ikoreshwa mukurema ibinyabuzima byahinduwe genetike (GMOs).
Gutegura Isomero Rikurikirana: PCR igira uruhare mugutegura amasomero ya ADN ya tekinoroji ikurikiranye. Ifasha kwagura ibice bya ADN kumurongo wo hasi ukurikirana.
Urubuga ruyobowe na Mutagenezi: PCR ikoreshwa mugutangiza ihinduka ryihariye muburyo bwa ADN, bituma abashakashatsi biga ingaruka zimpinduka zishingiye ku moko.
Urutoki rwa ADN: PCR ikoreshwa muburyo bwa ADN bwo gutunga urutoki kugirango bamenye umuntu ku giti cye, gupima ba se, no gushiraho umubano w’ibinyabuzima.
Ikiranga
• Kugaragara neza, ubunini bworoshye, nuburyo bukomeye.
• Bifite ibikoresho-byo hejuru, bicecekeye bya axial-flux kugirango ibikorwa bituje.
• Ibiranga imikorere yagutse ya 30 ℃, itanga uburyo bwiza bwo kugerageza ibintu byujuje ibisabwa byubushakashatsi.
• 5-santimetero ndende-isobanura amabara ya touchscreen kugirango ikorwe neza kandi yoroshye, igushoboza guhindura bitagoranye, kuzigama, no gukora progaramu.
• Sisitemu yo mu rwego rwinganda, yorohereza ibikorwa bikomeza kandi bitarimo amakosa 7x24.
• Ihererekanyamakuru ryihuse kuri USB flash ya disiki kugirango yorohereze porogaramu, yongere ubushobozi bwo kubika amakuru.
• Ikoranabuhanga rigezweho ryo gukonjesha hamwe nubuhanga budasanzwe bwo kugenzura ubushyuhe bwa PID buzamura imikorere muri rusange murwego rwo hejuru: kugenzura ubushyuhe bukabije, ubushyuhe bwihuse no gukonjesha, hamwe nubushyuhe bwa module imwe.
Ibibazo
Ikibazo: Umukinnyi wamagare yumuriro ni iki?
Igisubizo: Umukinnyi wamagare yumuriro nigikoresho cya laboratoire ikoreshwa mugukomeza ADN cyangwa RNA ikurikirana binyuze mumurongo wa polymerase (PCR). Ikora mukugenda mumagare binyuze murukurikirane rwubushyuhe, bituma ADN ikurikirana.
Ikibazo: Nibihe bintu nyamukuru bigize umukinnyi wamagare yumuriro?
Igisubizo: Ibyingenzi byingenzi bigize cycle yumuriro harimo guhagarika ubushyuhe, gukonjesha amashanyarazi, ibyuma byubushyuhe, microprocessor, hamwe ninama yo kugenzura.
Ikibazo: Nigute umukinnyi wamagare yumuriro akora?
Igisubizo: Umukinnyi wamagare yubushyuhe akora ashyushya no gukonjesha ingero za ADN murukurikirane rwubushyuhe. Inzira yo gusiganwa ku magare ikubiyemo gutandukana, guhuza, no kwagura ibyiciro, buri kimwe gifite ubushyuhe bwihariye nigihe bimara. Izi nzinguzingo zituma urutonde rwa ADN rwiyongera cyane binyuze muri polymerase yerekana (PCR).
Ikibazo: Ni ibihe bintu by'ingenzi ugomba gusuzuma mugihe uhisemo gusiganwa ku magare?
Igisubizo: Bimwe mubintu byingenzi ugomba gusuzuma mugihe uhisemo gusiganwa ku magare yumuriro harimo umubare wamariba cyangwa imiyoboro ya reaction, igipimo cyubushyuhe n'umuvuduko ukabije, uburinganire n'ubwuzuzanye bwo kugenzura ubushyuhe, hamwe nubukoresha hamwe nubushobozi bwa software.
Ikibazo: Nigute ushobora kubungabunga amagare yumuriro?
Igisubizo: Kugirango ukomeze gusiganwa ku magare yumuriro, ni ngombwa guhora usukura ibyuma bishyushya hamwe nigituba cya reaction, kugenzura niba ibice byashize, hanyuma ugahindura ibyuma byerekana ubushyuhe kugirango harebwe neza ubushyuhe kandi buhoraho. Ni ngombwa kandi gukurikiza amabwiriza yabakozwe yo kubungabunga no gusana buri gihe.
Ikibazo: Ni izihe ntambwe zisanzwe zo gukemura ibibazo kumagare yumuriro?
Igisubizo. Ni ngombwa kandi kwerekeza ku mabwiriza yakozwe n'abashinzwe gukemura ibibazo byihariye.
