Gusunika Umwanya Gel Electrophoresis CHEF Mapper A4

Ibisobanuro bigufi:

CHEF Mapper A4 ikwiranye no kumenya no gutandukanya molekile ya ADN kuva kuri 100 bp kugeza 10 Mb. Harimo ishami rishinzwe kugenzura, icyumba cya electrophoreis, icyumba gikonjesha, pompe izenguruka, hamwe nibindi bikoresho.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Icyitegererezo	CHEF Mapper A4
Umuvuduko w'amashanyarazi	0.5V / cm kugeza kuri 9,6V / cm, byiyongereyeho 0.1V / cm
Ntarengwa	0.5A
Umuvuduko ntarengwa	350V
Inguni	0-360 °
Igihe cyagenwe	Umurongo
Guhindura Igihe	50m kugeza 18h
Igihe ntarengwa cyo kwiruka	999h
Umubare wa Electrode	24, bigengwa n'ubwigenge
Ubushyuhe	0 ℃ kugeza kuri 50 ℃, ikosa ryo gutahura <± 0.5 ℃

Ibisobanuro

Imashini isunika gel electrophoresis (PFGE) itandukanya molekile ya ADN ihinduranya umurima w'amashanyarazi hagati ya electrode itandukanye yerekanwe ahantu hatandukanye, bigatera molekile ya ADN, ishobora kuba miriyoni ebyiri zifatizo zifatizo ndende kandi ikimuka ikanyura mu byobo bya agarose ku muvuduko utandukanye. Igera ku myanzuro ihanitse muri uru rwego kandi ikoreshwa cyane muri biologiya yubukorikori; kumenya imiterere y'ibinyabuzima na mikorobe; ubushakashatsi muri epidemiologiya; ubushakashatsi bwibice binini bya plasmid; kwimenyekanisha kwa gen; gushushanya umubiri wa gen, gusesengura RFLP, no gutunga urutoki ADN; ubushakashatsi bwurupfu rwa selile; ubushakashatsi ku kwangiza no gusana ADN; kwigunga no gusesengura ADN genomic; gutandukanya ADN ya chromosomal; kubaka, kumenyekanisha, no gusesengura amasomero manini y'ibitabo; n'ubushakashatsi bwa transgenji.t kwibanda kuri 0.5 ng / µL (dsDNA).

Gusaba

Birakwiye kumenya no gutandukanya molekile ya ADN kuva kuri 100bp kugeza 10Mb mubunini, ikagera kumurongo mwinshi murwego.

Ikiranga

• Ikoranabuhanga rigezweho: Ihuza CHEF na PACE pulsed-field tekinoroji kugirango igere ku bisubizo byiza hamwe n'inzira igororotse, idahetamye.

• Igenzura ryigenga: Ibiranga 24 bigengwa na electrode ya platine yigenga (0.5mm diameter), hamwe na electrode isimburwa kugiti cye.

• Imikorere yo kubara mu buryo bwikora: Ihuza ibintu byinshi byingenzi bihinduka nka voltage gradient, ubushyuhe, inguni yo guhinduranya, igihe cyambere, igihe cyanyuma, igihe cyo guhinduranya, igihe cyo gukora cyose, voltage, hamwe nigihe cyo kubara byikora, bifasha abakoresha kugera kubintu byiza byubushakashatsi.

• Algorithm idasanzwe: Koresha algorithm idasanzwe yo kugenzura impiswi kugirango igire ingaruka nziza zo gutandukana, itandukanya byoroshye ADN y'umurongo n'izunguruka, hamwe no gutandukanya ADN nini izenguruka.

• Automation: Mu buryo bwikora yandika kandi igatangira electrophoreis niba sisitemu ihagaritswe kubera kunanirwa kw'amashanyarazi.

• Umukoresha-Kugena: Emerera abakoresha kwishyiriraho imiterere yabo.

• Ihinduka: Sisitemu irashobora guhitamo voltage yihariye hamwe nigihe cyo guhinduranya kubunini bwa ADN ingana.

• Mugaragaza Kinini: Ifite ecran ya 7-LCD ya ecran kugirango ikorwe byoroshye, igaragaramo kugenzura software idasanzwe kugirango ikoreshwe byoroshye kandi byoroshye.

• Kumenya Ubushyuhe: Ubushakashatsi bubiri bwubushakashatsi bwerekana ubushyuhe bwa buffer hamwe namakosa ari munsi ya ± 0.5 ℃.

• Sisitemu yo kuzenguruka: Iza ifite sisitemu yo kuzenguruka igenzura neza kandi ikanagenzura ubushyuhe bwumuti wa buffer, bigatuma ubushyuhe buhoraho hamwe nuburinganire bwa ionic mugihe cya electrophorei.

• Umutekano muke: Harimo igifuniko cyumutekano wa acrylic kibonerana gihita kigabanya ingufu mugihe cyazamuwe, hamwe nuburemere burenze kandi nta mirimo yo kurinda imitwaro.

• Kuringaniza Urwego: Ikigega cya electrophoreis na gel caster biranga ibirenge bishobora guhinduka kugirango uringanize.

Igishushanyo mbonera: Ikigega cya electrophoreis gikozwe hamwe nuburyo bubumbabumbwe budahujwe; amashanyarazi ya electrode afite ibikoresho bya 0.5mm bya platine electrode, byemeza igihe kirekire nibisubizo byubushakashatsi bihamye.

Ibibazo

Ikibazo: Niki Gisunika Field Gel Electrophoresis?

Igisubizo: Pulsed Field Gel Electrophoresis nubuhanga bukoreshwa mugutandukanya molekile nini za ADN ukurikije ubunini bwazo. Harimo guhinduranya icyerekezo cyumuriro wamashanyarazi muri materix ya gel kugirango ushobore gutandukanya ibice bya ADN binini cyane kuburyo bidashobora gukemurwa na gakondo ya agarose gel electrophorei.

Ikibazo: Ni ubuhe buryo bukoreshwa bwa Pulsed Field Gel Electrophoresis?

Igisubizo: Pulsed Field Gel Electrophoresis ikoreshwa cyane mubinyabuzima bwa molekuline na genetika ya:

Gushushanya molekile nini za ADN, nka chromosomes na plasmide.

• Kumenya ingano ya genome.

• Kwiga itandukaniro ryimiterere nubusabane bwihindagurika.

Epidemiologiya ya molecular, cyane cyane mugukurikirana indwara zandura.

• Isesengura ryangiritse rya ADN no gusana.

• Kumenya ko hari genes zihariye cyangwa ADN zikurikirana.

Ikibazo: Nigute Pulsed Field Gel Electrophoresis ikora?

Igisubizo. Ibi bituma molekile nini ya ADN ihinduka hagati ya pulses, igafasha kugenda binyuze muri materix ya gel. Molekile ntoya ya ADN igenda yihuta binyuze muri gel, mugihe nini nini igenda gahoro, bigatuma gutandukana kwabo gushingiye kubunini.

Ikibazo: Ni irihe hame riri inyuma ya Pulsed Field Gel Electrophoresis?

Igisubizo: Pulsed Field Gel Electrophoresis itandukanya molekile ya ADN ukurikije ubunini bwayo mugucunga igihe nicyerekezo cyumuriro wamashanyarazi. Umwanya uhinduranya utera molekile nini ya ADN guhora yisubiraho, biganisha ku kwimuka kwabo binyuze muri materix ya gel no gutandukana ukurikije ubunini.

Ikibazo: Ni izihe nyungu za Pulsed Field Gel Electrophoresis?

Igisubizo.

Ikibazo: Ni ibihe bikoresho bikenewe kuri Pulsed Field Gel Electrophoresis?

Igisubizo: Pulsed Field Gel Electrophoresis mubisanzwe ikenera ibikoresho bya electrophoreis hamwe na electrode yihariye yo kubyara imirima. Agarose gel matrix hamwe nibitekerezo bikwiye hamwe na buffer. Amashanyarazi ashoboye kubyara pulses nyinshi. Sisitemu yo gukonjesha kugirango ikwirakwize ubushyuhe butangwa mugihe cya electrophore, na pompe yo kuzenguruka.