SDS-PAGE Gel Sisitemu ya Electrophoresis

Ibisobanuro bigufi:

Electrophoresis ni tekinike ya laboratoire ikoresha amashanyarazi kugirango itandukane ADN, RNA cyangwa proteyine ukurikije imiterere yumubiri nkubunini nubunini. DYCZ-24DN ni selile ya vertical electrophoreis selile ishobora gukoreshwa kuri SDS-PAGE gel electrophorei. SDS-PAGE, izina ryuzuye ni sodium dodecyl sulfate - polyacrylamide gel electrophorei, ikunze gukoreshwa nkuburyo bwo gutandukanya poroteyine nimbaraga za molekile hagati ya 5 na 250 kDa. Nubuhanga bukoreshwa cyane mubinyabuzima, ibinyabuzima bya molekuline na biotechnologiya gutandukanya poroteyine ukurikije uburemere bwazo.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Ibisobanuro bya tekiniki kuri DYCZ-24DN
Igipimo (LxWxH)	140 × 100 × 150mm
Ingano ya Gel (LxW)	75 × 83mm
Comb	Iriba 10 n'iriba 15
Kurwanya Ubunini	1.0mm na 1.5mm（Bisanzwe）0,75mm (Bihitamo)
Umubare w'icyitegererezo	20-30
Umubumbe wa Buffer	400 ml
Ibiro	1.0kg
Ibisobanuro bya tekinike kuri DYY-6C
Igipimo (LxWxH)	315 x 290x 128mm
Umuvuduko w'amashanyarazi	6-600V
Ibisohoka Ibiriho	4-400mA
Imbaraga zisohoka	240W
Ibisohoka	Ibice 4 muburyo bubangikanye
Ibiro	5.0kg

Ibisobanuro

DYCZ - 24DN ni selile ntoya ya vertical electrophoresis selile ikoreshwa kuri protein electrophoreis, ikaba yoroshye, yoroshye kandi yoroshye gukora sisitemu. Yakozwe muri polyikarubone ndende hamwe na electrode ya platine. Sisitemu irimo umubiri nyamukuru (gel casting stand), umupfundikizo hamwe na sisitemu, tank yo hanze (tanker buffer) hamwe nibikoresho bya gel. Ibikoresho: isahani yikirahure, ibimamara, ikibaho kinini cyibirahure (∮ = 5 mm) kugirango ukore gel imwe, ikadiri idasanzwe. Itanga amariba 10 na 15 hamwe nuburebure bwa 1.0mm na 1.5mm, kandi itanga kandi ibimamara bidahwitse hamwe na 0,75mm yuburebure hamwe nicyapa cyibirahure cyometseho hamwe na regula (0,75 mm) kugirango uhitemo. Intangiriro yacyo idafite inshinge kandi yatewe inshinge ibonerana irinda kumeneka no kumeneka. Irashobora kubika igisubizo cya buffer, igisubizo gikora buffer ni hafi 170; ml 170 gusa yumuti wa buffer irashobora kurangiza igerageza. Sisitemu ifite umutekano cyane kubakoresha. Inkomoko yimbaraga zayo zizimya mugihe umukoresha afunguye umupfundikizo. Igishushanyo kidasanzwe cyumupfundikizo kirinda gukora amakosa.

DYY-6C ni amashanyarazi yagenewe amashanyarazi kugirango akore amashanyarazi kugirango atandukane na ADN / RNA, PAGE electrophoreis no kwimurira muri membrane. DYY-6C ishyigikira ibisohoka 400V, 400mA, na 240W. LCD yayo irashobora kwerekana voltage, ikigezweho, imbaraga nigihe cyigihe kimwe. Irashobora gukora muburyo buhoraho bwa voltage, cyangwa muburyo buhoraho bwumuriro wamashanyarazi, kandi bigahinduka byikora ukurikije ibipimo byateganijwe mbere kubintu bitandukanye.

Gusaba

DYCZ-24DN ifite amashanyarazi DYY-6C ni sisitemu ya electrophoreis ya SDS-PAGE cyangwa Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis yo gutandukanya poroteyine kandi ikoreshwa cyane mubice byubucamanza, genetiki, ibinyabuzima na biologiya. Abashakashatsi bavuga incamake zikurikira za SDS-PAGE:
1.Bikoreshwa mugupima uburemere bwa molekile.
2.Bikoreshwa mukugereranya ubunini bwa poroteyine.
3.Yakoreshejwe mugushushanya peptide
4.Bikoreshwa mukugereranya polypeptide yibigize bitandukanye.
5.Bikoreshwa mukugereranya ubuziranenge bwa poroteyine.
6.Bikoreshwa muri Western Blotting.
7.Bikoreshwa mugupimisha virusi itera sida kugirango batandukane poroteyine za sida.
8.Gusesengura ingano n'umubare wa polypeptide subunits.

Ikiranga

DYCZ-24DN ikozwe mubikoresho byiza byo mu mucyo, bifite isura nziza, byemerwa cyane nabakiriya bacu. Ifite ibintu bikurikira:
• Ikozwe muri polikarubone yujuje ubuziranenge, nziza kandi iramba, byoroshye kubireba;
• Hamwe na jel itera muburyo bwumwimerere, ibasha guterera no gukoresha gel ahantu hamwe, byoroshye kandi byoroshye gukora geles, kandi ukabika umwanya wawe w'agaciro;
Igishushanyo cyihariye cya wedge kirashobora gutunganya icyumba cya gel;
• Ikigega kibumbabumbwe gifite ibikoresho bya electrode nziza;
• Biroroshye kandi byoroshye kongeramo ingero;
• Ashoboye gukoresha gel imwe cyangwa geles ebyiri icyarimwe;
Kubika igisubizo cya buffer;
• Igishushanyo cyihariye cya tank wirinde kumeneka na gel;
• Gukuraho electrode, byoroshye kubungabunga no kweza;
• Auto-off-off mugihe umupfundikizo ufunguye;

DYY-6C nkibicuruzwa byacu bigurishwa bishyushye bifite voltage ihamye nubu. Ibikurikira nibintu byihariye:
• Micro-mudasobwa itunganya igenzura ryubwenge;
• Ashoboye guhindura ibipimo mugihe nyacyo mubikorwa byakazi;
• Kinini-nini ya LCD yerekana voltage, ikigezweho, imbaraga nigihe cyigihe kimwe.
• Umuvuduko, amashanyarazi hamwe nimbaraga zifunze-kugenzura, kumenya guhinduka mugihe gikora.
• Hamwe nimikorere yo gukira.
• Nyuma yo kugera ku gihe cyagenwe, ifite umurimo wo gukomeza imiyoboro mito.
• Kurinda neza nibikorwa byo kuburira hakiri kare.
• Hamwe nibikorwa byo kubika kwibuka.
• Imashini imwe ifite ibibanza byinshi, ibisubizo bine bisa.