Electrophoresis Yimura Byose-muri-Sisitemu

Ibisobanuro bigufi:

Sisitemu ya electrophoreis yimura byose-muri-sisitemu ni igikoresho cyagenewe kwimura poroteyine zitandukanijwe na elegitoronike ziva muri geli zijya muri membrane kugirango zisesengurwe. Imashini ikomatanya imikorere ya tank ya electrophoreis, gutanga amashanyarazi hamwe nogukwirakwiza muri sisitemu ihuriweho. Ikoreshwa cyane mubushakashatsi bwibinyabuzima bya molekuline, nko mu gusesengura imvugo ya poroteyine, uko ADN ikurikirana, hamwe n’iburengerazuba. Ifite ibyiza byo kubika umwanya, kugabanya umwanda, no koroshya inzira yubushakashatsi.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro

Ibisobanuro kuri Tank ya Electrophoresis
Ingano ya Gel (LxW)	83 × 73mm
Comb	Amariba 10 (Bisanzwe) Amariba 15 (Bihitamo)
Kurwanya Ubunini	1.0 mm (Bisanzwe) 0,75, 1,5 mm (Ihitamo)
Isahani ngufi	101 × 73mm
Isahani Ikirahure	101 × 82mm
Umubumbe wa Buffer	300 ml
Ibisobanuro kuri Transfer Module
Agace ka Blotting (LxW)	100 × 75mm
Umubare w'abafite Gel	2
Intera ya Electrode	4cm
Umubumbe wa Buffer	1200ml
Ibisobanuro kuri Electrophoresis Amashanyarazi
Igipimo (LxWxH)	315 x 290 x 128mm
Umuvuduko w'amashanyarazi	6-600V
Ibisohoka Ibiriho	4-400mA
Imbaraga zisohoka	240W
Ibisohoka	Ibice 4 muburyo bubangikanye

Ibisobanuro

Electrophoresis ihererekanya byose-muri-sisitemu igizwe na tank ya electrophoreis ifite umupfundikizo, amashanyarazi afite akanama gashinzwe kugenzura, hamwe na module yoherejwe hamwe na electrode. Ikigega cya electrophoreis gikoreshwa mu guta no gukoresha geles, kandi module yoherejwe ikoreshwa mu gufata gel na membrane sandwich mugihe cyo kwimura, kandi ifite agasanduku gakonje kugirango birinde ubushyuhe. Amashanyarazi atanga amashanyarazi akenewe kugirango akoreshe gel kandi atware ihererekanyabubasha rya molekile kuva muri geli yerekeza muri membrane, kandi ifite akanama gashinzwe kugenzura abakoresha mugushiraho amashanyarazi no kwimura ibintu. Ihererekanyabubasha ririmo electrode ishyirwa muri tank hanyuma igahura na gel na membrane, ikuzuza amashanyarazi akenewe kugirango yimurwe.

Electrophoresis yimura all-in-one sisitemu nigikoresho cyingenzi kubashakashatsi naba technicien bakorana na proteine ntangarugero. Igishushanyo mbonera cyayo kandi cyoroshye cyo kuyikoresha bituma kongerwaho agaciro muri laboratoire iyo ari yo yose igira uruhare mu binyabuzima cyangwa ibinyabuzima.

Gusaba

Electrophoresis yimura byose-muri-sisitemu nigikoresho cyagaciro mubijyanye na biologiya ya molekuline, cyane cyane mu isesengura rya poroteyine. Poroteyine zoherejwe noneho zimenyekana hakoreshejwe antibodi zihariye muburyo bwitwa Western blotting. Ubu buhanga butuma abashakashatsi bamenya poroteyine zihariye kandi bakagereranya urwego rwabo.

Ikiranga

• Igicuruzwabihuye n'ubunini buto PAGE gel electrophorei;

• Igicuruzwa's ibipimo, ibikoresho birahujwe rwose nibicuruzwa byingenzi biranga isoko;

•Imiterere igezweho nigishushanyo cyiza;

• Menya neza ingaruka nziza yo kugerageza kuva gel guta kugeza gel ikora;

•Kwihutira kwimura geles ntoya;

• Cassettes ebyiri zifata Gel zirashobora gushirwa muri tank;

• Irashobora kwiruka kugeza kuri geles 2 mu isaha imwe. Irashobora gukora nijoro kugirango yimure ubukana buke;

• Cassettes ya gel ifite amabara atandukanye yemeza neza.

Ibibazo

Ikibazo: Niki kwimura electrophoreis byose-muri-sisitemu ikoreshwa?

Igisubizo: Electrophoresis ihererekanya byose-muri-sisitemu ikoreshwa mu kwimura poroteyine ziva muri geli polyacrylamide kuri membrane kugirango zisesengurwe, nka Western blotting.

Ikibazo: Ni ubuhe bunini bwa gel bushobora gukorwa no kwimurwa hakoreshejwe electrophoreis yimura byose-muri-sisitemu?

Igisubizo: electrophoreis yimura all-in-one sisitemu irashobora guterera no gukoresha gel ingano ya 83X73cm yo gutera intoki, na 86X68cm mbere yo gutera. Agace koherejwe ni 100X75cm.

Ikibazo: Nigute electrophoreis yimura byose-muri-sisitemu ikora?

Igisubizo: Electrophoresis yimura all-in-imwe sisitemu ikoresha electrophoreis kugirango yimure poroteyine ziva muri gel zijya muri membrane. Poroteyine zabanje gutandukanywa nubunini hakoreshejwe polyacrylamide gel electrophorei (PAGE) hanyuma ikoherezwa muri membrane ikoresheje umurima w'amashanyarazi.

Ikibazo: Ni ubuhe bwoko bwa membrane bushobora gukoreshwa hamwe na electrophoreis yimura byose-muri-sisitemu?

Igisubizo: Ubwoko butandukanye bwa membrane burashobora gukoreshwa hamwe na electrophoreis ihererekanya byose muri sisitemu harimo nitrocellulose na PVDF (polyvinylidene difluoride).

Ikibazo: Ese electrophoreis yimura sisitemu-imwe-imwe ishobora gukoreshwa mu gusesengura ADN?

Igisubizo: Oya, Electrophoresis ihererekanya-imwe-imwe ya sisitemu yagenewe umwihariko wo gusesengura poroteyine kandi ntishobora gukoreshwa mu gusesengura ADN.

Ikibazo: Ni izihe nyungu zo gukoresha electrophoreis yimura sisitemu-imwe-imwe?

Igisubizo: Electrophoresis yimura sisitemu-imwe-imwe ituma ihererekanyabubasha rya poroteyine ziva muri geli zijya muri membrane, zitanga ibyiyumvo bihanitse kandi byihariye mu kumenya poroteyine. Nubundi buryo bworoshye-muri-sisitemu yorohereza inzira yuburengerazuba.

Ikibazo: Nigute electrophoreis yimura sisitemu-imwe-imwe igomba gukomeza?

Igisubizo: Electrophoresis yimura sisitemu-imwe-imwe isukurwa nyuma yo gukoreshwa hanyuma ikabikwa ahantu hasukuye, humye. Electrode nibindi bice bigomba kugenzurwa buri gihe niba byangiritse cyangwa kwambara.