Ni ubuhe buryo bwo gusiganwa ku magare muri PCR?

Polymerase Chain Reaction (PCR) ni tekinike ya biologiya ikoreshwa muguhuza ibice bya ADN byihariye. Irashobora gufatwa nkuburyo bwihariye bwo kwigana ADN hanze y’ibinyabuzima bizima. Ikintu nyamukuru kiranga PCR nubushobozi bwayo bwo kongera cyane urugero rwa ADN.

Incamake yumurongo wa Polymerase

Ishusho ya Bioninja

Ni ubuhe buryo bwo gusiganwa ku magare muri PCR?

PCR igizwe n'intambwe eshatu z'ibanze:

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2. Annealing ya Template ADN na Primers: Nyuma yo gutandukana, ubushyuhe buramanuka bugera kuri 55 ° C, bigatuma primers ihuza urukurikirane rwuzuzanya kuri ADN imwe imwe.

3. Kwagura Primers: Kuri 72 ° C, ADN yerekana icyitegererezo-primer ikomeza kwaguka hifashishijwe ADN polymerase (nka Taq ADN polymerase), ikoresheje dNTPs nka substrate kugirango ihuze umurongo mushya wuzuzanya ushingiye ku cyitegererezo cya ADN.

Beijing LIUYI Imashini ya PCR

Mugusubiramo gutandukana, guhuza, no kwagura inzira, hashyirwaho "igice cya kabiri cyo kwigana igice", gishobora kuba nk'icyitegererezo cyizunguruka. Buri cyiciro gifata iminota igera kuri 2 kugeza kuri 4, bigatuma gene igenewe kwongerwaho miriyoni inshuro mumasaha 2 kugeza kuri 3.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ifite ubuhanga mu gukora ibikoresho bya electrophoreis mu myaka irenga 50 hamwe nitsinda ryacu ryubuhanga ryumwuga hamwe nikigo cya R&D. Dufite umurongo wizewe kandi wuzuye kuva mubishushanyo kugeza kugenzura, hamwe nububiko, hamwe ninkunga yo kwamamaza. Ibicuruzwa byacu byingenzi ni Akagari ka Electrophoresis (tank / chamber), Electrophoresis Amashanyarazi, Ubururu LED Transilluminator, UV Transilluminator, Gel Image & Analyse Sisitemu nibindi kandi dutanga ibikoresho bya laboratoire nkibikoresho bya PCR, mixer ya vortex na centrifuge ya laboratoire.

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira. Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.

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