ADN Gel Electrophoresis: Gusesengura ibice bya genetike

ADN gel electrophorei ni tekinike ya biologiya isanzwe ikoreshwa mugutandukanya no gusesengura ibice bya ADN ukurikije ubunini bwabyo.Inzira ikubiyemo gupakira ibice bitandukanye bya ADN kuri jel ikozwe muri agarose, karubone ya hydrata iboneka muri algae itukura.

Gutegura no gutera agarose gel

1. Kuramo agarose muburyo bukwiye bwa buffer ya electrophoreis.Ubwinshi bwa gel bugenwa nuburinganire-bwinshi, nka 1g ya agarose muri 100ml ya buffer kuri gel 1%.
2. Shyushya imvange muri microwave, uzenguruke kontineri kugirango agarose irangire.
3. Ongeramo etidium bromide kumuti wa gel kumurongo wanyuma wa 0.5mg / ml.Ethidium bromide ihuza hagati ya ADN yegeranye kandi ikanasohora fluorescence munsi yumucyo UV.Menya ko etidium bromide ari kanseri, kubwibyo rero bisaba ingamba zumutekano zikwiye nko kwambara gants.
4. Hisha igisubizo cya gel mu bwogero bwamazi kugirango wirinde inzira ya gel gutemba kubera ubushyuhe bwinshi.
5. Shira ikimamara mu gisubizo cya gel kugirango ukore amariba y'icyitegererezo.Hitamo ibimamara bikwiranye nubunini bwa ADN y'icyitegererezo uzaba urimo gupakira.Suka agarose gel igisubizo murigel traykandi ubemerera gukomera mubushyuhe bwicyumba.
6. Gele imaze gukomera, kura ibimamara.Niba utazahita ukoresha gel, uyizingire muri plastike hanyuma ubibike kuri 4 ℃ kugeza bikenewe.

Gutegura no gukoresha gel

Mbere yo gutangira electrophoreis, vanga icyitegererezo cya ADN hamwe na bffer yipakurura.Ububiko bwo gupakira busanzwe bwikubye inshuro esheshatu kandi bufasha icyitegererezo kurohama munsi yamariba no gukurikirana urujya n'uruza mugihe cya electrophorei.

Shiraho amashanyarazi kuri voltage yagenwe.

Ongeramo buffer ya electrophoreis ihagije kuri tank ya gel kugirango utwikire hejuru ya gel.Menya nezaguhuza neza electrode.

Shyiramo ADN icyitegererezo hamwe nuburemere bwa molekuline mubiriba bya gel.

Zimya amashanyarazi kugirango utangire amashanyarazi.

Kwitegereza ibice bya ADN bitandukanye

Nyuma ya electrophoreis, uzimye amashanyarazi hanyuma ukureho gel.

Koresha urumuri rwa UV kugirango umurikire gel;Ibice bya ADN bizagaragara nka bande ya orange fluorescent.

Andika ishusho ya gel kugirango usesengure ibice bya ADN bitandukanye.

Nyuma yubushakashatsi, menya neza ko wajugunywe neza ya gel na electrophoreis, ukurikiza amabwiriza ya laboratoire.Buri gihe ujye wambara uturindantoki mugihe ukoresha geles na buffer zirimo etidium bromide kugirango wirinde guhura nibi bintu byangiza.

ADN gel electrophorei ikoreshwa cyane mubinyabuzima bwa molekuline no mubushakashatsi bwerekeranye na genetike mugupima ingano ya molekile ya ADN, gutandukanya ibice bya ADN, kumenya ihinduka ryimiterere ya gene, no gutunga urutoki rwa ADN, mubindi bikorwa.Nubuhanga bworoshye kandi bunoze bwubushakashatsi bufasha mugusobanukirwa imiterere nibiranga ingero za ADN.

Beijing Liuyi Biotechnology Co.Ltd, uruganda rukora umwuga wibanda ku bicuruzwa bya electrophoreis mu myaka 50, rutanga ibigega bitandukanye bya electrophoreis (chambers / selile) kuri gel electrophorei, nka tanki ya electrophoreis ya horizontal (chambre / selile) kuriADNgel electrophorei, hamwe na vertical electrophoresis tanks (chambers / selile) kuri protein gel electrophorei.Hagati aho, itanga kandi ubwoko butandukanye bwa electrophoreis itanga amashanyarazi, abaministri b'umwijima wisesengura hamwe na UV transilluminator.Urashobora guhitamo ibigega bya electrophoreis ya Beijing Liuyi (chambers / selile) kugirango upime uburemere bwa molekuline ya RNA no gutandukanya RNA yubunini butandukanye kuri laboratoire yawe.

Hano tuzabikorasabaubwoko bumwe bwa horizontal electrophoresis tank (chamber / selile) icyitegererezoDYCP-31DN gukora no gukoresha gel.

Nyuma yo gukoresha gel, urashobora gukoresha ishusho yacu ya gel & isesengura rya sisitemuWD-9413Bkwitegereza, gusesengura no gufata amashusho ya gel.Liuyi Biotech itanga kandi UV transilluminator (UV Analyser) kugirango yitegereze gel.Dufite umukara-agasanduku ubwoko bwa UV transilluminator (UV Analyser)WD-9403A,9403C,WD-9403F, portable UV transilluminator (UV Analyser) icyitegererezoWD-9403Bno gufata UV transilluminator (UV Analyser)WD-9403Ekuko wahisemo.

Ikirango cya Beijing Liuyi gifite amateka yimyaka irenga 50 mubushinwa kandi isosiyete irashobora gutanga ibicuruzwa bihamye kandi byujuje ubuziranenge kwisi yose.Binyuze mumyaka yiterambere, birakwiye guhitamo!

Ubu turimo gushakisha abafatanyabikorwa, ikigega cya OEM electrophoresis hamwe nababitanga barahawe ikaze.

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira.Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.