Polyacrylamide ikoreshwa kenshi mubinyabuzima bya molekuline nkibikoresho bya electrophoreis ya proteyine na acide nucleic muri tekinike izwi nka PAGE.Nubwoko bwa zone electrophoreis yuburyo bwa synthetics gel bita polyacrylamide nkibikoresho bifasha.Yubatswe na S.Raymond na L.Weintraub mu 1959, hanyuma izamurwa kandi itezwa imbere na L.Ornstein na BJ Davis.Ubu buryo bwakoreshejwe cyane nyuma yo kubisobanura no kubihindura nabo mubitekerezo na tekinike yubushakashatsi muri 1964.
Mbere yo gukoresha polyacrylamide gel electrophorei, abantu bakoresha cyane amashanyarazi electrophoreis kuri zone EP.Ariko impapuro zikora nkigikoresho, gusa zifite imikorere ya anti-convection, ntayindi ngaruka nziza.Mugihe gel ya polyacrylamide idafite imikorere ya anti-convection gusa ahubwo irashobora no kwitabira gutandukana ikomeza.Kuberako gel polyacrylamide ni ubwoko bwimiterere ya net, aribwo polymerisation no guhuza guhuza acrylamide (Acr) na N, N-methylenebis (acrylamide).Acrylamide yitwa monomer, mugihe N, N-methylenebis yitwa comonomer cyangwa crosslinker.Gukora gel ni inzira ya polymerisiyumu.Ingano ya pore ya jel irashobora kugenzurwa, bityo gel irashobora gukorwa hamwe nimpamyabumenyi zitandukanye.Niba ingano ya pore iri hafi ya radiyo ngereranyo ya molekile yintangarugero, kurwanya molekile kunyura muri pore ya gel bizagira isano ya hafi nubunini n'imiterere ya molekile mugihe cya electrophorei.Itanga rero impinduka ihinduka yo gutandukanya ibyo bikoresho hamwe ninshingano zisa.
Hariho inzira ebyiri rusange zikoreshwa kuri polyacrylamide gel electrophorei, imwe ni disiki ya electrophorei, indi ni slab electrophorei.Icyapa cya electrophoreis cyakozwe muburyo bwihariye bwo gutandukanya poroteyine na ADN, kandi muri rusange, hari ubwoko bubiri bwa slab electrohporesis, aribwo tank ya horizontal electrophoreis na tank ya vertical electrophoreis.Kuri poroteyine, abantu bakoresha ikigega cya electrophorei ya horizontal kuri IFF na immunoelectrophoresis, bitabaye ibyo, abantu bakoresha ibigega bya vertical electrophoreis kuri proteine.
Beijing Liuyi Biotechnology ifite ubwoko butandukanye bwibikoresho bya plaque electrophoreis ya PAGE, usibye gusesengura no kumenya intangarugero za poroteyine na polyacrylamide gel electrophorei, irashobora kandi gukoreshwa mugupima uburemere bwa molekile, kweza ingero no gutegura ingero.
Fata icyitegererezoDYCZ-23Ank'urugero, ni ikigega gisanzwe cya vertical electrophoreis ya Laboratoire.Koresha amasahani abiri yikirahure kugirango ukore icyumba cya gel kugirango ukore gel, hanyuma ukome amashyi amasahani yikirahure kugirango wirinde gutemba.Umubyimba wa gel uterwa nubunini bwa spacer.Mubisanzwe, umubyimba ni 1.5mm kugirango urekure neza, kandiDYCZ-23Aitanga kandi uburebure bwa 1.0mm bwo guta gel 1.0mm.Usibye ikigega cya electrophoreis, kugirango ukore electrophoreis, amashanyarazi nayo arakenewe.Beijing Liuyi Biotechnology itanga ibintu bitandukanyeamashanyarazi.Kuva kuri voltage ndende kugeza kuri voltage yo hasi, uhitamo icyitegererezo ukurikije porogaramu.
Ikirango cya Beijing Liuyi gifite amateka yimyaka irenga 50 mubushinwa kandi isosiyete irashobora gutanga ibicuruzwa bihamye kandi byujuje ubuziranenge kwisi yose.Binyuze mumyaka yiterambere, birakwiye guhitamo!
Ubu turimo gushakisha abafatanyabikorwa, ikigega cya OEM electrophoresis hamwe nababitanga barahawe ikaze.
Kubindi bisobanuro kuri twe, nyamuneka twandikire ukoresheje imeri[imeri irinzwe]cyangwa[imeri irinzwe].
Igihe cyo kohereza: Ukwakira-12-2022