Poroteyine Electrophoresis Ibibazo Bisanzwe

Ibibazo bya poroteyine ya electrophoreis bivuga ibibazo cyangwa ibitagenda neza bishobora kuvuka mugihe cyo gutandukanya poroteyine ukurikije umuriro w'amashanyarazi ukoresheje amashanyarazi.Ibi bibazo birashobora kuba birimo isura yimigozi itunguranye cyangwa idasanzwe, gukemura nabi, gusiga, cyangwa kugoreka imirongo ya poroteyine, nibindi.Turasobanura muri make ibibazo byinshi bikunze kugaragara kubakiriya bacu kugirango tumenye impamvu no kubona ibisubizo nyabyo no kuzamura ireme rya protein.

Kumwenyuraband- bande yerekana imirongo igana hejuru kumpande zombi za gel

Impamvu
1. Hagati ya gel ikora ishyushye kuruta impera zombi
2. Imiterere yububasha burenze

Igisubizo
① Buffer ntabwo ivanze neza cyangwa buffer mucyumba cyo hejuru cyane.Ongera usubiremo buffer, urebe neza kuvanga neza, cyane cyane iyo ugabanije ububiko bwa 5x cyangwa 10x
Kugabanya ingufu ziva kuri 200 V kugeza kuri 150 V cyangwa kuzuza icyumba cyo hasi kugeza muri cm 1 hejuru yisahani ngufi

Inzira ya poroteyine

Impamvu
1. Icyitegererezo kiremereye
2. Icyitegererezo cy'imvura

Igisubizo
Gukuramo icyitegererezo, hitamo gukuramo poroteyine yiganje muri sample, cyangwa kugabanya voltage hafi 25% kugirango ugabanye umurongo
② Centrifuge sample mbere yo kongeramo SDS sample buffer, cyangwa kugabanya% T ya gel
Ikigereranyo cya SDS na poroteyine kigomba kuba gihagije cyo gutwika buri molekile ya poroteyine na SDS, muri rusange 1.4: 1.Irashobora gusaba SDS nyinshi kuri proteine ​​zimwe na zimwe

Bnamu buryo butambitsegukwirakwira

Impamvu
1. Gutandukanya amariba mbere yo gufungura ikigezweho
2. Ionic imbaraga zicyitegererezo kiri munsi yizya gel

Igisubizo
Gabanya igihe kiri hagati yicyitegererezo no gufungura imbaraga zo gutangira
② Koresha buffer imwe murugero nko muri gel cyangwa gel

Intungamubiri za poroteyine zigoretse cyangwa zigoramye

Impamvu
1. Polimerizasi mbi ikikije amariba
2. Umunyu murugero
3. Imigaragarire ya gel itaringaniye

Igisubizo
Degas gutondekanya gel igisubizo mbere yo gukina;ongera ammonium persulfate hamwe na TEMED yibanze kuri 25%, kugirango ushyire gel cyangwa hasi% T, usige APS kimwe kandi wikubye kabiri TEMED.
Kuraho umunyu ukoresheje dialyse, kuyungurura;
Kugabanya igipimo cya polymerisation.Gupfundikanya geles witonze.

Beijing Liuyi Biotechnology Company Ltd ikora ibicuruzwa bitandukanye bya electrophoreis bishobora gufasha gukemura ibibazo wenda duhura nabyo mubushakashatsi bwa electrophoreis.

Beijing Liuyi Biotechnology Company Ltd yashinzwe mu 1970, yahoze yitwa Uruganda rukora ibikoresho bya Beijing Liuyi, ni uruganda rukora ibikoresho bya laboratoire n'ibikoresho bya siyansi bifite icyicaro i Beijing mu Bushinwa.Hamwe nuburambe bwimyaka irenga 50 mugushushanya no gukora ibicuruzwa bya electrophoreis nubushakashatsi bwa siyanse yubuzima, ibaye umuyobozi wambere utanga ibikoresho bya laboratoire nibisubizo mubushinwa. Ibicuruzwa byikigo birimo ibikoresho byinshi bya laboratoire, harimo ikigega cya nucleic acide electrophoreis, proteine ​​vertical ikigega cya electrophoreis / unit, umukara-agasanduku ubwoko bwa UV isesengura, Gel Document Tracking Imaging Analyser, hamwe namashanyarazi.Ibicuruzwa bikoreshwa cyane mubigo byubushakashatsi, kaminuza, ninganda zitandukanye, nka farumasi, ibinyabuzima, no gukurikirana ibidukikije. Isosiyete ni ISO9001 & ISO13485 isosiyete yemewe kandi ifite ibyemezo bya CE.

Harihoubwoko butandukanye bwauhagaritseibigega bya electrophoreisprotein electrophoreisyo gusesengura no kumenya intangarugero za poroteyine na polyacrylamide gel electrophorei,nano gupima uburemere bwa molekuline uburemere, kweza ingero no gutegura ingero.Ibicuruzwa byose biremewe muriisoko ryo mu gihugu no hanze.

Amashanyarazi ya electrophoreis nikintu cyingenzi cyasisitemu ya electrophoreis, gutanga isoko ihamye kandi yuzuye yumuriro wamashanyarazi kugirango utware inzira yo gutandukana.Itmubisanzwe itanga voltage ihoraho cyangwa ihoraho kuri sisitemu ya electrophoreis, bitewe na protocole yihariye yubushakashatsi.Iyemerera kandi uyikoresha guhindura voltage cyangwa ibisohoka muri iki gihe, kimwe nibindi bipimo nkigihe nubushyuhe, kugirango ahindure imiterere yo gutandukana kubushakashatsi runaka.

Fcyangwa kwitegereza gel, urashobora guhitamo UV Transilluminator WD-9403 yakozwe na Beijing Liuyi Biotechnology.A UV transilluminator nigikoresho cya laboratoire ikoreshwa mugushushanya no gusesengura ADN, RNA, na proteine.Ikora mu kumurika ibyitegererezo hamwe nurumuri rwa UV, itera ingero za fluoresce kandi zigaragara.Hariho moderi nyinshi za UV transilluminatoryatanzwe nawe.WD-9403A ni umwihariko wo kureba poroteyine electrophorei, kandi WD-9403F ikoreshwa mu kureba ADN na poroteyine electrophorei.

Uru ruhererekane rw'ibicuruzwa rushobora gutanga kuva kuri casting kugeza kwitegereza gel ukurikije ibyo usabwa gukora.Tumenyeshe ibyo usabwa, OEM, ODM hamwe nababigenewe murakaza neza.We tuzagerageza uko dushoboye kugirango tuguhe ibicuruzwa na serivisi.

Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira.Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.