Twasangiye ibitekerezo byinshi mucyumweru gishize cyo gukoresha selile ya acetate membrane electrophoresis, kandi tuzasoza iyi ngingo hano uyumunsi kugirango uyikoreshe.
Guhitamo Kwibanda kwa Buffer
Ububiko bwa buffer bukoreshwa muri selilose acetate membrane electrophoreis muri rusange ni munsi ugereranije nibikoreshwa mumpapuro za electrophorei.Bikunze gukoreshwa pH 8.6Bbuffer ya arbital isanzwe ihitamo murwego rwa 0.05 mol / L kugeza 0.09 mol / L.Iyo uhisemo kwibanda, hafatwa icyemezo kibanza.Kurugero, niba uburebure bwumurongo wa membrane hagati ya electrode mucyumba cya electrophoreis ari 8-10cm, ingufu za 25V zisabwa kuri santimetero z'uburebure bwa membrane, kandi ubukana bwubu bugomba kuba 0.4-0.5 mA kuri santimetero z'ubugari bwa membrane.Niba izo ndangagaciro zitagerwaho cyangwa zirenze mugihe cya electrophorei, kwibanda kwa buffer bigomba kwiyongera cyangwa kuvangwa.
Kurenza urugero cyane ya buffer bizavamo umuvuduko wihuta wa bande no kwiyongera mubugari.Ku rundi ruhande, kwibanda cyane kuri buffer bizagabanya umuvuduko wimuka, bigatuma bigora gutandukanya imirongo imwe yo gutandukana.
Twabibutsa ko muri selulose acetate membrane electrophoresis, igice kinini cyumuyaga gikorwa hifashishijwe icyitegererezo, gitanga ubushyuhe butari buke.Rimwe na rimwe, ihitamo rya buffer ryatoranijwe rishobora gufatwa nkibikwiye.Nubwo bimeze bityo ariko, mubihe byubushyuhe bwibidukikije bwiyongera cyangwa mugihe ukoresheje ingufu nyinshi, guhumeka kwamazi bitewe nubushyuhe birashobora kwiyongera, bikaviramo kwibanda cyane kuri buffer ndetse bigatuma na membrane ikama.
Icyitegererezo
Muri selulose acetate membrane electrophoresis, ingano yubunini bwikitegererezo igenwa nibintu bitandukanye, harimo imiterere ya electrophoreis, imiterere yicyitegererezo ubwacyo, uburyo bwo gusiga, hamwe nubuhanga bwo gutahura.Nka ihame rusange, nuburyo bworoshye bwo kumenya uburyo bwo kumenya, ntoya urugero ntangarugero rushobora kuba, rufite akamaro ko gutandukana.Niba urugero rwicyitegererezo ari rwinshi, uburyo bwo gutandukanya amashanyarazi ntibushobora kuba busobanutse, kandi irangi rishobora no gutwara igihe.Nyamara, mugihe cyo gusesengura muburyo butandukanye imirongo yatandukanijwe ikoresheje uburyo bwa elution colorimetric detection, ingano yicyitegererezo ntigomba kuba nto cyane, kuko ishobora kuvamo indangagaciro zo kugabanuka kubintu bimwe, biganisha kumakosa menshi mukubara ibirimo.Mu bihe nk'ibi, urugero rw'icyitegererezo rugomba kongerwa mu buryo bukwiye.
Mubisanzwe, ingano yicyitegererezo yongewe kuri buri santimetero yumurongo wicyitegererezo wasabye kuva kuri 0.1 kugeza 5 μL, bihwanye nicyitegererezo kingana na 5 kugeza 1000 μg.Kurugero, mubisanzwe isesengura rya poroteyine electrophoreis isesengura, ingano yicyitegererezo yongewe kuri buri santimetero yumurongo wasabye muri rusange ntabwo irenga 1 μL, ihwanye na 60 kugeza 80 μg za poroteyine.Ariko, mugihe usesenguye lipoproteine cyangwa glycoproteine ukoresheje uburyo bumwe bwa electrophoreis, ingano yicyitegererezo igomba kwiyongera.
Mugusoza, urugero rwiza rwicyitegererezo rugomba gutoranywa hashingiwe kumiterere yihariye binyuze murukurikirane rwubushakashatsi.
Guhitamo Igisubizo
Imirongo yatandukanijwe muri selile ya acetate membrane electrophoresis isanzwe yanduye mbere yo gutahura.Ibice bitandukanye byicyitegererezo bisaba uburyo butandukanye bwo gusiga, kandi uburyo bwo gusiga bukwiranye na selile ya acetate ya membrane electrophoreis ntishobora gukoreshwa rwose kurupapuro.
Hariho amahame atatu yingenzi yo guhitamo igisubizo kiboneyeselulose acetate membrane.Ubwa mbere,Irangi ryangirika ryamazi rigomba guhitamo kuruta irangi ryokunywa inzoga kugirango wirinde kugabanuka kwa membrane no guhinduka biterwa nigisubizo cyanyuma.Nyuma yo kwanduza, ni ngombwa koza amazi hamwe n'amazi no kugabanya igihe cyo kumara.Bitabaye ibyo, ururenda rushobora guhinduka cyangwa kugabanuka, byagira ingaruka kubimenyekana nyuma.
Icya kabiri, nibyiza guhitamo amarangi afite irangi rikomeye kuri sample.Muri selulose acetate membrane electrophoresis ya proteine za serumu, amino black 10B ikoreshwa cyane kubera kuba ifite irangi ryinshi kubintu bitandukanye bya poroteyine za serumu kandi bihamye.
Icya gatatu, amabara meza yizewe agomba guhitamo.Amabara amwe, nubwo afite izina rimwe, arashobora kuba arimo umwanda bivamo umwijima mwinshi nyuma yo kwanduza.Ibi birashobora no kuvanga umwimerere utandukanijwe neza, bigatuma bigorana gutandukanya.
Ubwanyuma, guhitamo ibisubizo byibanda kumurongo ni ngombwa.Mu buryo bw'igitekerezo, birasa nkaho urwego rwo hejuru rwibisubizo byakemuwe byatera kwanduza neza ibice byintangarugero nibisubizo byiza.Ariko, ntabwo aribyo.Guhuza isano hagati yicyitegererezo hamwe n irangi bifite imipaka runaka, itiyongera hamwe no kwiyongera kwibisubizo byibisubizo.Ibinyuranye na byo, igisubizo kirenze urugero cyibisubizo byibanze ntibitesha irangi gusa ahubwo binagorana kugera kumateka asobanutse.Byongeye kandi, iyo ubukana bwamabara bugeze kumurongo ntarengwa ntarengwa, umurongo wogusiga irangi ntukurikiza umurongo ugaragara, cyane cyane mubipimo byo kubara.Mu selulose acetate membrane electrophoresis, kwibanda kumurongo wibisubizo biri munsi ugereranije nibikoreshwa mumashanyarazi.
Ibisobanuro kugirango umenye ibya Beijing Liuyi Ibinyabuzima's selulose acetate membraneikigega cya electrophoreis hamwe nikoreshwa rya electrophoreis, nyamuneka sura hano:
lUbushakashatsi bwo gutandukanya serumu proteyine na Cellulose Acetate Membrane
lCellulose Acetate Membrane Electrophoresis
Niba ufite gahunda yo kugura ibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira.Urashobora kutwoherereza ubutumwa kuri imeri[imeri irinzwe]cyangwa[imeri irinzwe], cyangwa nyamuneka uduhamagare kuri +86 15810650221 cyangwa wongere Whatsapp +86 15810650221, cyangwa Wechat: 15810650221.
Reba:Electrophoresis (integuro ya kabiri) na Bwana Li
Igihe cyo kohereza: Jun-06-2023